Human Islets for Diabetes Research and Transplantation - LIDSEN Publishing Inc.丨The Open Access Publisher

Special Issue

Human Islets for Diabetes Research and Transplantation

Submission Deadline: March 31, 2019 (Open) Submit Now

Guest Editor

Tatsuya Kin, MD, PhD

Clinical Islet Laboratory, University of Alberta Hospital, 210 College Plaza, 8215-112 St, Edmonton, Alberta, Canada T6G2C8

Website | E-Mail

Research Interests: Islet isolation; islet transplantation

About the Topic

The past decade has seen enormous progress in clinical islet transplantation as a therapy for type 1 diabetes, as well as steady expansion in human islet isolation activity worldwide. Islet isolation centers have been providing human islets for clinical transplantation and for basic research. Thanks to improvement of islet isolation technology and implementation of islet distribution programs, many basic researchers now have access to this valuable resource. In the face of recent recognition that human islets differ in a critical way from nonhuman counterparts, the demand of human islets for basic research is ever increasing and novel findings on human islet biology are accumulating. Also cells derived from human pancreatic tissue have a potential for future development of cellular based therapy for diabetes. The aim of this issue is to serve as a platform to update the current knowledge and recent progress in the basic research utilizing human islets as well as in clinical islet transplantation.

Publication

Open Access Short Report

Estradiol 17-β Induces Pancreatic Beta-Cell Proliferation through Distinct Estrogen Receptors in a Glucose Dependent Manner

Sigal Shaklai ,

Meital Grafi-Cohen ,

Orli Sharon ,

Gabi Shefer ,

Dalia Somjen and

Naftali Stern

Received: 17 April 2019; Published: 27 August 2019; doi: 10.21926/obm.transplant.1903082

Abstract

Background: Estradiol 17-beta (E2) enhances the function and survival of pancreatic beta-cells but its clinical use has been questioned due to concerns regarding oncogenic potential and feminizing effects in males. The G-protein coupled estrogen receptor (GPER), expressed in pancreatic islets, exhib [...]

Open Access Research Article

Immunoprotective Encapsulation of Micro-Organs

Assaf Assis ,

Lena Sapozhnikov ,

Sandra Camargo and

Eduardo Mitrani

Received: 07 May 2019; Published: 14 August 2019; doi: 10.21926/obm.transplant.1903078

Abstract

Background: Cell encapsulation technology is most likely the ultimate solution for autologous cell therapy based clinical approaches. A key issue when developing a functional encapsulated construct, is to consider not only the nature of the capsule but also how the cells should be incorporated into [...]

Open Access Review

Our Steps toward Subcutaneous Transplantation of Macro-Encapsulated Islets

Shoichiro Sumi ,

Sin-Yu Yang ,

Priyadarshini Canning and

Kai-Chiang Yang

Received: 29 May 2019; Published: 24 July 2019; doi: 10.21926/obm.transplant.1903074

Abstract

Background: Diabetes mellitus (DM) can be cured or greatly ameliorated by adequate insulin secretion from a relatively small volume of insulin-producing cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. However, micro-encapsulated islets used in rec [...]

Open Access Review

Single Cell Transcriptomic Analysis of Pancreatic β Cell Development and Differentiation from Pluripotent Stem Cells

Chunhui Xie ,

Yeh-Chuin Poh ,

Lillian Ye and

George Harb

Received: 06 May 2019; Published: 17 July 2019; doi: 10.21926/obm.transplant.1903072

Abstract

Single cell genomics is a powerful tool to study cellular heterogeneity and discover novel cell types. Recent studies used single cell RNA sequencing (scRNA-seq) to analyze the transcriptomes of individual pancreatic islet cells. Islets are a complex mixture of endocrine cells and therefore represen [...]

Open Access Research Article

A Simple and Effective Non-Human Primate Diabetic Model Combining Sub-Total Pancreatectomy and Low-Dose Streptozotocin Injection

Ibrahim Fathi ,

Takehiro Imura ,

Kozue Imura ,

Megumi Goto ,

Yasuhiro Igarashi ,

Akiko Inagaki ,

Makiko Kikkawa ,

Fumiko Ono ,

Hiroo Iwata ,

Yohichi Yasunami and

Masafumi Goto

Received: 27 March 2019; Published: 09 July 2019; doi: 10.21926/obm.transplant.1903071

Abstract

BACKGROUND. Current non-human primate diabetic models, namely those induced by total pancreatectomy or streptozotocin (STZ) injection, suffer from several disadvantages, including a long surgical procedure, the need for continual digestive enzyme replacement, and a delayed oral intake after total pa [...]

Open Access Research Article

The DPP4 Inhibitor Sitagliptin Increases Active GLP-1 Levels from Human Islets and May Increase Islet Cell Survival Prior to Transplantation

Scott A. Campbell ,

Matthew Hubert ,

Janyne Johnson ,

Nicole Salamon and

Peter E. Light

Received: 12 March 2019; Published: 26 June 2019; doi: 10.21926/obm.transplant.1902069

Abstract

Background One of the goals of clinical islet transplantation is to achieve a single-donor transplant that is dependent on obtaining enough quality  cell mass from one donor pancreas. Human islets are routinely cultured prior to transplantation, and pro-survival factors such as GLP-1 analogues have [...]

Open Access Review

Human Islet Isolation and Distribution Efforts for Clinical and Basic Research

Natasha Ng ,

Wei Xuan Tan ,

Yexin Koh and

Adrian Teo

Received: 11 March 2019; Published: 21 June 2019; doi: 10.21926/obm.transplant.1902068

Abstract

The ability to obtain purified human islets routinely and reproducibly has enabled substantial progress in providing a safe and reliable treatment option for adult Type 1 diabetes patients. The availability of human islets for basic research has also significantly impacted the progress of understand [...]

Open Access Original Research

Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation

Gopalakrishnan Loganathan ,

Michael G. Hughes ,

Gregory L. Szot ,

Kate E. Smith and

Appakalai N. Balamurugan

Received: 29 March 2019; Published: 24 May 2019; doi: 10.21926/obm.transplant.1902064

Abstract

Background: Purified bacterial collagenase and protease enzymes are commonly used to recover human islets from clinical grade pancreata for subsequent clinical islet transplantation. The high cost of purified enzymes can be prohibitive for recovering human islets from research pancreata used for tra [...]

Open Access Editorial

Pancreatic Islet Transplantation: State of the Art and Future Perspectives

Gianluca Donatini ,

S Giraud ,

JL Kraimps and

T Hauet

Received: 08 April 2019; Published: 15 April 2019; doi: 10.21926/obm.transplant.1902062

Abstract

Pancreatic islet transplantation represents an effective therapy with lower morbidity for patients carriers of type 1 diabetes compared to whole pancreas transplantation. Although complete insulin independence is usually not achieved it allows control of glycemia balance reducing the risk of severe [...]

Open Access Review

Islet Autotransplantations for Total Pancreatectomy

Naoaki Sakata ,

Gumpei Yoshimatsu and

Shohta Kodama

Received: 15 January 2019; Published: 03 April 2019; doi: 10.21926/obm.transplant.1902059

Abstract

Total pancreatectomy (TP) is performed for not only malignant pancreatic diseases but also benign disease like chronic pancreatitis or pancreatic injury. However, it always abolishes the pancreatic exo- and endocrine functions and the nutritional condition of the patients is significantly impaired. [...]