Development of a Novel Pipette Tip-Aided Cell Cloning Method for The Effective Isolation of Genome-Edited Porcine Cell
Abstract
1941 11131
Development of a Novel Pipette Tip-Aided Cell Cloning Method for The Effective Isolation of Genome-Edited Porcine CellAbstract
Direct colony cloning of adherent mammalian cells using rings or dilution cloning has been used frequently for obtaining stable transfectants after gene delivery. As an alternative to these methods, successful isolation of the cells in a single colony is possible by placing a trypsin-immersed small paper disk onto the colony and subsequently picking up the paper with the assumption that it carries the trypsinized cells. However, the cloning success using this technique largely relies on the cell type used. In the p [...] 1941 11131 |
Partial Response of Alopecia Areata to Helicobacter Pylori Eradication and Treatment with Low-Dose Naltrexoneby
Abstract
Alopecia areata is an autoimmune disease of unknown origin. It has been proposed that it is a multifactorial disease with genetic, immunological, infectious, circulatory, and psychogenic factors being involved in its etiopathogenesis. Its association with other autoimmune diseases is frequent. The infection of Helicobacter pylori has been linked to multiple immuno-mediated extradigestive conditions including alopecia areata. There is scientific evidence of the usefulness of naltrexone in low doses (an opioid antago [...] 1413 11127 |
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Open Access Short Communication Newborn Screening in Latin America: A Window on the Evolution of Health PolicyAbstract
Investigating the historical origins of a well-accepted health program across a region, such as newborn screening in Latin America, has the potential to reveal the role of historically-specific drivers in shaping national health policy. We reviewed published reports on NBS in Latin America and interviewed newborn screening experts with experience in the region. We found trends suggesting that the decrease in infant mortality may be a prerequisite to investing in nationwide screening for rarer conditions. We also no [...] 1418 11123 |
Application of Clinical Next Generation Sequencing in Intensive Care Facilitates Rapid Diagnosis of Neonates with Rare Genetic Disordersby
Abstract
Background: Neonatal muscular hypotonia is a common clinical feature on neonatal intensive care units with a broad spectrum of etiologies. Besides more common and obvious underlying conditions like prematurity or Down syndrome many rare disorders with often poor prognosis need to be considered. Congenital brain malformations detected on ultrasound or/and magnetic resonance imaging might constitute the clinical hallmark in differential diagnosis. We report on our approach combining cerebral imaging and application o [...] 1896 11118 |
Scientific Evidence in Homeopathy: A Dynamic DatabaseAbstract
Homeopathy, a system of Traditional and Complementary Medicine (T&CM, according to WHO definition), is under frequent attack by skeptics because of its supposed “lack of evidence.” To overcome the distrust of skeptics and the public, many databases have been created, which focused on collecting all the published and indexed studies; however, none of these seem to be “comprehensive” and systematic enough. Therefore, this study aimed to evaluate the most reliable and available homeopathic studies in order to systemat [...] 1290 11118 |
A Large Eddy Simulation Study of Cyclones: The Effects of Interparticle Collisions on Erosion Predictionby
Abstract
Cyclone separators are widely used in fluid catalytic cracking (FCC) units due to their lack of moving parts and relatively low-pressure drop. However, cyclone separators are prone to erosion-related issues, which is a major drawback. In this paper, a large eddy simulation (LES) of the particle-gas flow in a cyclone separator is investigated using a four-way Euler-Lagrange approach to model inter-particle collisions and the exchange of momentum between particles and fluid. The effects of inter-particle and particle [...] 1695 11115 |
Moderators of Treatment Outcomes from Family Caregiver Skills Training: Secondary Analysis of a Randomized Controlled Trialby
Abstract
Background: Moderator analyses may be helpful for evaluating intervention effects. The objective of this study was to evaluate whether the effect of a caregiver skills training intervention – Helping Invested Families Improve Veterans' Experience Study (HI-FIVES) – on care recipient outcome and caregiver outcome is moderated by the veteran’s risk for hospitalization or level of functional impairment. Methods: Secondary data analysis of HI-FIVES. Outcomes included veteran days in the community (cumulative days in th [...] 1954 11113 |
Newborn Screening for Inborn Errors of MetabolismAbstract
Newborn screening can now detect more than 50 disorders, providing early and often life-saving treatment. Inborn errors of metabolism account for the majority of these disorders. This review will consider the more common metabolic disorders identified on newborn screening, including history, technique and management of these disorders. 1581 11111 |
Our Steps toward Subcutaneous Transplantation of Macro-Encapsulated IsletsAbstract
Background: Diabetes mellitus (DM) can be cured or greatly ameliorated by adequate insulin secretion from a relatively small volume of insulin-producing cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. However, recent clinical trials show that micro-encapsulated islets are not fully retrievable after transplantation. By contrast, macro-encapsulated islets can be retrieved when necessary. As to the transplantation site, subcutaneous tissue can be promising, if new [...] 1483 11105 |
A Review on the Ethnomedicinal Plants Used in Zimbabwe for the Treatment and Management of Skin Conditions: Perspectives on Pharmacological and Toxicological Evaluationby
Abstract
Medicinal plants remain central to healthcare in Africa, where up to 80% of the population relies on traditional remedies to manage a range of health conditions, including skin ailments. In Zimbabwe, the country's rich biodiversity and favourable agroecological zones support a diverse range of ethnomedicinal plants. However, much of the knowledge surrounding these plants remains dispersed and scientifically under-evaluated. This review consolidates evidence from ethnobotanical surveys, peer-reviewed research, and g [...] 1426 11102 |
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