Table of Content

Open Access Communication

Development of a Novel Pipette Tip-Aided Cell Cloning Method for The Effective Isolation of Genome-Edited Porcine Cell

Received: 11 October 2020;  Published: 10 March 2021;  doi: 10.21926/obm.genet.2101126

Abstract

Direct colony cloning of adherent mammalian cells using rings or dilution cloning has been used frequently for obtaining stable transfectants after gene delivery. As an alternative to these methods, successful isolation of the cells in a single colony is possible by placing a trypsin-immersed small paper disk onto the colony and subsequently picking up the paper with the assumption that it carries the trypsinized cells. However, the cloning success using this technique largely relies on the cell type used. In the p [...]

1940 11130

Open Access Case Report

Partial Response of Alopecia Areata to Helicobacter Pylori Eradication and Treatment with Low-Dose Naltrexone

Received: 26 June 2019;  Published: 30 September 2019;  doi: 10.21926/obm.hg.1903037

Abstract

Alopecia areata is an autoimmune disease of unknown origin. It has been proposed that it is a multifactorial disease with genetic, immunological, infectious, circulatory, and psychogenic factors being involved in its etiopathogenesis. Its association with other autoimmune diseases is frequent. The infection of Helicobacter pylori has been linked to multiple immuno-mediated extradigestive conditions including alopecia areata. There is scientific evidence of the usefulness of naltrexone in low doses (an opioid antago [...]

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Open Access Short Communication

Newborn Screening in Latin America: A Window on the Evolution of Health Policy

Received: 22 August 2021;  Published: 21 January 2022;  doi: 10.21926/obm.genet.2201146

Abstract

Investigating the historical origins of a well-accepted health program across a region, such as newborn screening in Latin America, has the potential to reveal the role of historically-specific drivers in shaping national health policy. We reviewed published reports on NBS in Latin America and interviewed newborn screening experts with experience in the region. We found trends suggesting that the decrease in infant mortality may be a prerequisite to investing in nationwide screening for rarer conditions. We also no [...]

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Open Access Original Research

Application of Clinical Next Generation Sequencing in Intensive Care Facilitates Rapid Diagnosis of Neonates with Rare Genetic Disorders

Received: 31 August 2017;  Published: 06 March 2018;  doi: 10.21926/obm.genet.1801015

Abstract

Background: Neonatal muscular hypotonia is a common clinical feature on neonatal intensive care units with a broad spectrum of etiologies. Besides more common and obvious underlying conditions like prematurity or Down syndrome many rare disorders with often poor prognosis need to be considered. Congenital brain malformations detected on ultrasound or/and magnetic resonance imaging might constitute the clinical hallmark in differential diagnosis. We report on our approach combining cerebral imaging and application o [...]

1896 11118

Open Access Review

Scientific Evidence in Homeopathy: A Dynamic Database

Received: 27 April 2021;  Published: 28 September 2021;  doi: 10.21926/obm.icm.2103032

Abstract

Homeopathy, a system of Traditional and Complementary Medicine (T&CM, according to WHO definition), is under frequent attack by skeptics because of its supposed “lack of evidence.” To overcome the distrust of skeptics and the public, many databases have been created, which focused on collecting all the published and indexed studies; however, none of these seem to be “comprehensive” and systematic enough. Therefore, this study aimed to evaluate the most reliable and available homeopathic studies in order to systemat [...]

1290 11117

Open Access Original Research

A Large Eddy Simulation Study of Cyclones: The Effects of Interparticle Collisions on Erosion Prediction

Received: 31 January 2022;  Published: 06 May 2022;  doi: 10.21926/jept.2202017

Abstract

Cyclone separators are widely used in fluid catalytic cracking (FCC) units due to their lack of moving parts and relatively low-pressure drop. However, cyclone separators are prone to erosion-related issues, which is a major drawback. In this paper, a large eddy simulation (LES) of the particle-gas flow in a cyclone separator is investigated using a four-way Euler-Lagrange approach to model inter-particle collisions and the exchange of momentum between particles and fluid. The effects of inter-particle and particle [...]

1695 11115

Open Access Original Research

Moderators of Treatment Outcomes from Family Caregiver Skills Training: Secondary Analysis of a Randomized Controlled Trial

Received: 22 February 2019;  Published: 18 April 2019;  doi: 10.21926/obm.geriatr.1902049

Abstract

Background: Moderator analyses may be helpful for evaluating intervention effects. The objective of this study was to evaluate whether the effect of a caregiver skills training intervention – Helping Invested Families Improve Veterans' Experience Study (HI-FIVES) – on care recipient outcome and caregiver outcome is moderated by the veteran’s risk for hospitalization or level of functional impairment. Methods: Secondary data analysis of HI-FIVES. Outcomes included veteran days in the community (cumulative days in th [...]

1954 11113

Open Access Review

Newborn Screening for Inborn Errors of Metabolism

Received: 14 June 2023;  Published: 18 October 2023;  doi: 10.21926/obm.genet.2304197

Abstract

Newborn screening can now detect more than 50 disorders, providing early and often life-saving treatment. Inborn errors of metabolism account for the majority of these disorders. This review will consider the more common metabolic disorders identified on newborn screening, including history, technique and management of these disorders.

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Open Access Review

Our Steps toward Subcutaneous Transplantation of Macro-Encapsulated Islets

Received: 29 May 2019;  Published: 24 July 2019;  doi: 10.21926/obm.transplant.1903074

Abstract

Background: Diabetes mellitus (DM) can be cured or greatly ameliorated by adequate insulin secretion from a relatively small volume of insulin-producing cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. However, recent clinical trials show that micro-encapsulated islets are not fully retrievable after transplantation. By contrast, macro-encapsulated islets can be retrieved when necessary. As to the transplantation site, subcutaneous tissue can be promising, if new [...]

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Open Access Original Research

Dynamic Functional Connectivity Fading Analysis and Classification of Alzheimer’s Disease, Mild Cognitive Impairment and Normal Control Subjects based on Resting-State fMRI Data

Received: 29 September 2019;  Published: 01 June 2020;  doi: 10.21926/obm.neurobiol.2002059

Abstract

In this paper, motivated by the fading effect in wireless communications, where severe channel fading is related to information loss during the transmission, we evaluate and analyze the fading effect in time-varying functional connectivity of AD, MCI and NC subjects based on the resting-state fMRI data, and then apply that for AD, MCI, NC classification. We show that in some critical brain regions, compared with NC subjects, AD subjects suffer more severe and long lasting fading in the functional connectivity level [...]

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