by Adayabalam S. Balajee

Received: 26 August 2018;  Published: 19 October 2018;  doi: 10.21926/obm.genet.1804042

The technique of in situ hybridization (ISH) using radioactively labeled DNA probes was first described in the late 1960s and early 1970s. The first use of fluorescence in situ hybridization (FISH) was reported in 1980s where RNA labeled with a fluorophore at the 3’ end was used to detect specific DNA sequences. Since then, the technique has undergone various modifications for detecting single genes, chromosomes and whole genomes on various targets such as interphase nucleus, prematurely condensed chromosomes [...]

by Uttayan Chakrabarti  , Manish Chaturvedy  , Nitin Kumar Bajpai  , Jitendra Goswami  , Rajesh Kumar Garsa  and Rajesh Jhorawat

Received: 10 April 2023;  Published: 14 July 2023;  doi: 10.21926/obm.transplant.2303192

BK virus (BKV) is a common opportunistic pathogen in kidney transplant recipients and one of the most challenging causes of allograft dysfunction and loss. Although over-immunosuppression remains the primary risk factor for BKV infection after transplantation, male gender, older recipient age, prior rejection episodes, degree of human leukocyte antigen mismatching, prolonged cold ischemia time, BK virus serostatus and ureteral stent placement have all been implicated as risk factors. Routine screening post-renal tr [...]

by Tracy Harwood  , John Maltby  and Elizabeta B. Mukaetova-Ladinska

Received: 28 March 2019;  Published: 15 July 2019;  doi: 10.21926/obm.geriatr.1903062

Background: Art enhances both physical and mental health wellbeing. The health benefits include reduction in blood pressure, heart rate, pain perception and briefer inpatient stays, as well as improvement of communication skills and self-esteem. In addition to these, people living with dementia benefit from reduction of their non-cognitive, behavioural changes, enhancement of their cognitive capacities and being socially active. Methods: The current study represents a narrative general literature review on availabl [...]

by Asaad G Ishak  , Sundar V Cherukuri  , GianCarlo Diaz  and Richard McCallum

Received: 25 January 2021;  Published: 02 July 2021;  doi: 10.21926/obm.geriatr.2103174

Dysphagia, or difficulty swallowing, is a common condition, especially among the elderly population and can become debilitating. As we age, there are subtle changes in esophageal motility, but by no means is dysphagia ever considered to be a normal result of aging. Dysphagia usually presents with a variety of accompanying symptoms ranging from regurgitation during meals, mild discomfort, to severe chest pain. It can be challenging to localize the dysphagia from the history. The underlying etiology may become eviden [...]

by Khalid Saidov  , Anna Tiunova  , Oksana Subach  , Fedor Subach  and Konstantin Anokhin

Received: 12 October 2018;  Published: 04 March 2019;  doi: 10.21926/obm.genet.1901065

Background: The method of cellular compartment analysis of temporal activity by fluorescent in situ hybridization (catFISH) is widely used in cellular and behavioral neurobiology. This technique exploits stimulus-induced expression of immediate early genes (IEGs) and allows identification of two neuronal populations activated in the brain of the same animal in response to neural or behavioral events separated by 25–30 min. The differential labeling is based on the visualization of nuclear RNA and cytoplasmic [...]

by Em V. Adams  , Brandi M. Crowe  , Jasmine Vanadore  , Marieke Van Puymbroeck  and Arlene A. Schmid

Received: 06 February 2022;  Published: 22 April 2022;  doi: 10.21926/obm.icm.2202020

The purpose of this descriptive survey was to explore how, and for what purpose, health care practitioners use yoga in clinical practice. A survey was administered to professionals who identified as healthcare practitioners who used yoga in their practice. There were 205 completed responses from a combination of healthcare practitioners including occupational therapists, physical therapists, yoga therapists, recreational therapists, speech-language pathologists, and mental health counselors. Practitioners worked in [...]

by Cathryn Duchette  , Danilo V Tolusso  , Whitley J Stone  , Maire M Blankenship  and Rachel A Tinius

Received: 23 June 2021;  Published: 25 November 2021;  doi: 10.21926/obm.icm.2104051

Studies have demonstrated that new and expectant mothers experience increased levels of stress and anxiety during the COVID-19 pandemic. Though prenatal yoga is an effective mode of improving mental health during pregnancy, no research has evaluated its effect on mental health during times of extreme stress, such as a global pandemic. The purpose of this study was to determine the influence of a single session and a 10-week prenatal yoga intervention on the mental health of pregnant women during the COVID-19 pandem [...]

by Ronald J. Pekala  and Kevin Creegan

Received: 20 January 2020;  Published: 13 April 2020;  doi: 10.21926/obm.icm.2002019

Noetic analysis is a methodology to quantify the mind during hypnosis, meditation, or other stimulus conditions/states of consciousness in a reliable and valid manner. The methodology uses retrospective phenomenological assessment (RPA) to comprehensively assess subjective experience. By having the participant complete a first person, self-report questionnaire, the Phenomenology of Consciousness Inventory (PCI), in reference to a short stimulus condition, the researcher can generate a “snapshot” of the mind, and it [...]

by Olorunjuwon O. Bello  , Rosemary O. Akande  , Temitope K. Bello  , Muibat O. Fashola  , Mathew O. Oni  and Adeleke Osho

Received: 21 July 2024;  Published: 20 January 2025;  doi: 10.21926/obm.icm.2501006

The incidence of antifungal-resistant pathogenic fungi has steadily increased around the globe and calls for an aggressive response. The study aimed to produce and evaluate virgin coconut oil's phytochemical properties and antifungal potentials (VCO). The production of VCO was achieved by hot extraction and natural fermentation methods. Gas Chromatography-Mass Spectrometry (GC-MS) was used to evaluate the active phytochemicals in the VCO. The antifungal activities of VCO against Aspergillus flavus, Aspergillus nige [...]

by Karen Harrison Dening  and Zena Aldridge

Received: 26 February 2019;  Published: 10 May 2019;  doi: 10.21926/obm.geriatr.1902053

There has been a strong thrust to identify the needs of people with dementia against a background of person-centred care, however, people with dementia do not exist in isolation; they live within relational contexts with family members. Case management is a model of care that has its origins in supporting patients with a long term disease, however, a very practical approach to case management that can support the care needs of both the person with dementia and the family carers is emerging in the UK. Admiral Nursin [...]