by Georgia Kakourou  , Thalia Mamas  , Christina Vrettou  and Jan Traeger-Synodinos

Received: 03 June 2019;  Published: 22 July 2019;  doi: 10.21926/obm.genet.1903084

Preimplantation Genetic Testing for HLA-matching (PGT-HLA) has been one of the most controversial PGT applications, first reported in 2001. The procedure aims to identify an embryo that is not only healthy but also HLA-matched with a sibling in the family in need of haematopoietic stem cell transplantation (HSCT), considering that sibling HSCT stands the highest chance of success in comparison to alternative approaches. HLA-typing can be performed with or without PGT-M for the exclusion of a single-gene disorder. T [...]

by Mojtaba Ehsanifar  and Zeinab Yavari

Received: 13 December 2024;  Published: 16 March 2025;  doi: 10.21926/obm.neurobiol.2501277

The pervasive presence and enduring existence of micro and nanoplastics in the environment render their exposure to humans and aquatic creatures unavoidable. Research indicates these tiny plastic particles can be taken in by aquatic beings and mammals. Once within the body, micro and nanoplastics have the capability to infiltrate the brain, although the level of penetration and the subsequent neurotoxic effects are not fully explored. Previous studies indicate that metal (oxide) nanoparticles can enter the brain an [...]

by Kamal A.R. Ismail  , Fatima A.M Lino  , Jorge R. Henriquez  , Mohamed Teggar  , Abdelghani Laouer  , Muslum Arici  , Amani Benhorma  and Daniel Rodríguez

Received: 12 June 2023;  Published: 19 October 2023;  doi: 10.21926/jept.2304031

The building sector is rated as a big consumer of electric energy and emissions, responsible for about 40% of final electric energy consumption. As a result, the Paris Agreement 2015 set a goal for buildings and the construction sector to reach a nearly zero-carbon stage by 2050. This urged most countries to create regulations for the construction sector and invest in energy efficiency programs. The present paper aims to present an updated review of building energy-saving solutions and techniques to contribute to c [...]

by Mahintaj Dara  , Mehdi Dianatpour  , Negar Azarpira  and Nader Tanideh

Received: 04 September 2024;  Published: 08 January 2025;  doi: 10.21926/obm.genet.2501280

Gene therapy, a groundbreaking method for addressing genetic mutations, includes strategies such as gene repair, replacement, inactivation, or the introduction of therapeutic genes, circumventing traditional surgical or pharmacological approaches. Delivery through viral or non-viral vectors presents trade-offs in efficiency and immune response. Recent gene-editing technologies like ZFNs, TALENs, and CRISPR facilitate precise genome modifications by inducing targeted double-strand breaks, with CRISPR/Cas9 recognized [...]

by Samskruthi Menashi  , Lawrence B Cohen  and Manuela G. Neuman

Received: 10 February 2018;  Published: 28 March 2018;  doi: 10.21926/obm.icm.1801002

Ayurveda is an ancient Indian medicinal practice. The present review includes translational research characterizing obesity, non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). We reviewed published research on improving function through Ayurvedic medicine by searching electronic databases (Medline, Embase) using terms such as: Ayurveda, obesity, NAFLD, NASH, the effect of exercise interventions in obesity, change in body composition. We aim to (1) determine the immuno-pathology of [...]

by Scarlett Puebla-Barragan  , Britney Lamb  , Serenah Jafelice  and Gregor Reid

Received: 16 June 2021;  Published: 01 November 2021;  doi: 10.21926/obm.icm.2104040

Vaginal care products are widely used by women to relieve discomfort such as pain, itching and malodour, all of which are commonly caused by conditions resulting from microbiota dysbiosis. Previous studies showed that probiotic strains Lacticaseibacillus (formerly Lactobacillus) rhamnosus GR-1 (LGR-1) and Limosilactobacillus (formerly Lactobacillus) reuteri RC-14 (LRC-14), can aid in restoring homeostasis in the vaginal microbiome when taken orally. A topical product containing these strains could be of value for r [...]

by Masahiro Sato  , Issei Saitoh  , Yuki kiyokawa  , Eri Akasaka  , Shingo Nakamura  , Satoshi Watanabe  and Emi Inada

Received: 21 January 2022;  Published: 11 April 2022;  doi: 10.21926/obm.genet.2202151

Adipose tissue is distributed throughout the body as fat depots. The amount of adipose tissue increases with age. In mice, epididymal fat depots in males and gonadal fat depots in females are associated with the reproductive system. Regarding fat depots in females, the adipose tissue under the skin can be easily exposed via surgery when the ovary, oviduct, and uterus are pulled out and exposed. As handling adipose tissue is relatively easy, adipocytes might be good targets for genetic manipulation (including gene d [...]

by Zhenya Tang  , Jun Gu  , Guilin Tang  and L. Jeffrey Medeiros

Received: 22 July 2018;  Published: 03 October 2018;  doi: 10.21926/obm.genet.1804038

Because of its’ simplicity, reliability and cost-effectiveness, fluorescence in situ hybridization (FISH) is a major technology that is widely applied in clinical diagnosis, especially for hematologic malignancies, even in the era of next-generation sequencing (NGS). In the Clinical Cytogenetics Laboratory at MD Anderson Cancer Center, over 15,000 FISH tests are performed each year, including approximately 1,000 BCR-ABL1 and 500 MYC FISH tests, respectively. In this chapter, we introduce the quality assurance/quali [...]

by Cherechi Ndukwe  , M. Tariq Iqbal  and Jahangir Khan

Received: 16 November 2021;  Published: 17 February 2022;  doi: 10.21926/jept.2201007

SCADA provides real-time system monitoring by constant communication and data exchange between various system devices to achieve data visualization and logging. Presently, in industrial systems, commercial SCADA systems are being used for data monitoring and control. These systems can be expensive, and as such can only be afforded by select industries. Even at these costs, the commercial SCADA systems face some challenges, which include interoperability and scalability issues. Research has shown that these problems [...]

by Hong Yong Sohn  , Mohamed Elzohiery  and De-Qiu Fan

Received: 28 June 2021;  Published: 23 September 2021;  doi: 10.21926/jept.2103042

This article describes the development of a novel ironmaking technology based on flash reduction. The development started with the proof of the kinetic feasibility, considering that a typical flash reactor provides only a few seconds of residence time. This was followed by tests in a laboratory flash reactor and finally a pilot plant operation. The rate equations formulated in this work were developed considering the optimum combination of temperature, residence time, and reducing gas partial pressure to achieve &# [...]