Recent Progress in Nutrition is an international peer-reviewed Open Access journal published quarterly online by LIDSEN Publishing Inc. This periodical is devoted to publishing high-quality papers that describe the most significant and cutting-edge research in all areas of nutritional sciences. Its aim is to provide timely, authoritative introductions to current thinking, developments and research in carefully selected topics. Also, it aims to enhance the international exchange of scientific activities in nutritional science and human health.

Recent Progress in Nutrition publishes high quality intervention and observational studies in nutrition. High quality systematic reviews and meta-analyses are also welcome as are pilot studies with preliminary data and hypotheses generating studies. Emphasis is placed on understanding the relationship between nutrition and health and of the role of dietary patterns in health and disease.

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There is no restriction on paper length, provided that the text is concise and comprehensive. Authors should present their results in as much detail as possible, as reviewers are encouraged to emphasize scientific rigor and reproducibility.

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Free Publication in 2022
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Open Access Review

The Potential Role of Commensal Microbes in Optimizing Nutrition Care Delivery and Nutrient Metabolism

Anikka M. Swaby , Luis B. Agellon *

School of Human Nutrition, McGill University, Ste. Anne de Bellevue, QC, H9X 3V9, Canada

Correspondence: Luis B. Agellon

Academic Editor: Jennifer Keogh

Special Issue: Feature Papers of Recent Progress in Nutrition 2021

Received: December 20, 2021 | Accepted: May 20, 2022 | Published: May 26, 2022

Recent Progress in Nutrition 2022, Volume 2, Issue 2, doi:10.21926/rpn.2202014

Recommended citation: Swaby AM, Agellon LB. The Potential Role of Commensal Microbes in Optimizing Nutrition Care Delivery and Nutrient Metabolism. Recent Progress in Nutrition 2022; 2(2): 014; doi:10.21926/rpn.2202014.

© 2022 by the authors. This is an open access article distributed under the conditions of the Creative Commons by Attribution License, which permits unrestricted use, distribution, and reproduction in any medium or format, provided the original work is correctly cited.

Abstract

Microbes have been part of the diet throughout human history. In the evolution of food preservation practices, some techniques inadvertently leveraged microbial activity not only to extend the storage life but also to enhance the properties and nutritive value of foods. In the last century, a variety of bacterial species (referred to as probiotics) were found to confer health benefits to the host. The advent of high-throughput sequencing methods facilitated improved surveillance of conventional probiotics within gut microbial communities as well as fueled the deep exploration of the human gut microbiota. Metagenomic analyses along with improvements in microbial culture techniques and comprehensive functional characterization of specific microbes both in vitro and in vivo have shed new insights into the intimate relationship of the gut microbiota and its host. Recent findings suggest the potential of conventional and newly identified bacterial species in enhancing nutrient processing and holds promise in improving the efficacy of conventional nutrition intervention strategies in managing diseases as well as in the delivery of personalized nutrition therapy support.

Keywords

Diet; gut microbiota; microbiome; nutrient metabolism; nutrition care; personalized nutrition therapy; probiotics; synbiotics

1. Introduction

Microbes have long-standing symbiotic relationships with humans. Our body is colonized by different classes of microbes including archaea, fungi, viruses and bacteria [1]. Depending on the conditions and nutrient availability, different sets of microbes, collectively called microbiota, thrive in different body sites, and the gut microbiota is the largest and most complex collection [1,2]. Most of the gut microbial community consists of trillions of bacterial cells that represent thousands of different species of bacteria primarily belonging to one of the two dominant phyla, Firmicutes or Bacteroidetes [2]. As with other species with a gastrointestinal tract, humans have a characteristic gut microbiota composition. Acquired from the mother at birth, the human gut microbiota is shaped by intrinsic factors, such as biological sex, and extrinsic factors, such as infant feeding practices and environment, until a stable functional core population is established by early childhood [3,4]. Gut microbes actively participate in the maturation of the immune system and contribute to human nutrition by synthesizing essential vitamins (e.g., vitamin B12, vitamin K), processing the components of food and inhibiting the growth of pathogens [5,6,7]. The gut microbiota can be viewed as an accessory organ that processes both the nutrient and non-nutrient components of food and produces bioactive metabolites that play critical roles in moderating human nutrition and physiological processes [5,7,8].

The relationship between gut microbes and humans is complex and dynamic. The human gastrointestinal tract provides habitat and resources for microbes allowing a diverse community to thrive and execute functions that benefit both itself and its host. On the other hand, the host must regulate the microbial load and composition of this community which is partly accomplished through innate immunity that was programmed shortly after birth [9,10]. Some metabolites produced and secreted by the host (e.g., bile acids, hydrogen peroxide) can kill or inhibit the growth of microbes to help control the size of the microbial community and to maintain greater proportions of commensal members than pathogenic ones [11]. However, the composition of the community is also susceptible to alteration by host dietary habits which can result in either beneficial or pathogenic effects on the host. This intimate and bidirectional relationship can be manipulated to optimize the beneficial contributions of the gut microbiota to the host.

The study of human-associated microbes has yielded many important milestones in understanding the direct and indirect impacts of gut microbes on human heath [1,5]. Here we focus our discussion on the relationship among humans, nutrients and gut microbes and highlight recent research that demonstrates the important roles of specific commensal gut microbes in supporting human health. We also discuss recent developments in gut microbiota-targeted therapies and provide an outlook on how these approaches can potentially be leveraged to optimize nutrition care delivery and nutrient metabolism.

2. A Brief History of Probiotics

Microbes have been an integral part of the human diet. The need to process and preserve foods gave rise to early practices such as salting, drying and, notably, fermentation. During the fermentation process, microbes grow and metabolize available nutrients, producing a spectrum of metabolites [12]. Controlled fermentation renders foods more digestible since macronutrients are pre-processed by the microbes. The process also generates an array of compounds that impart new flavors which can enhance food palatability [13]. On the other hand, uncontrolled fermentation can spoil foods or induce illness and even death [14]. Interestingly, early humans inadvertently benefitted from the consumption of live microbes in a variety of fermented foods [12,15]. However, the concept of intentional administration of live microbes for health benefits, only emerged in the early 1900’s and credited to Elie Metchnikoff who proposed an association between the consumption of fermented foods and health [16,17]. Some scholars have disputed this attribution since Józef Brudziński performed earlier experiments using Bacillus lactis aërogenes to treat children with acute infectious diarrhea [17,18]. Around the same time, Henry Tissier also observed low bifidobacteria in the stool of children with diarrhea compared to those without and suggested that providing high amounts of this bacterium to affected children could alleviate the condition [19,20,21]. Both lactobacilli and bifidobacteria were postulated to be “beneficial” bacteria which drew the attention of researchers aiming to further study these organisms. The term probiotic is now used to describe microbes that, when ingested in sufficient amounts, confers a health benefit to the host [19,22].

Pioneering research to identify and characterize conventional probiotic candidates primarily relied on knowledge of how to grow these microorganisms in the laboratory. This entailed the use of nutrient-rich growth media that could ensure the growth of the microbe of interest in culture. Today, advanced tools and techniques such as high throughput sequencing platforms, advanced in vitro culturing systems and germ-free animals facilitate the deep exploration of the human gut microbiota. Metagenomic analysis enables the determination of the microbe genomes (including those that cannot be cultured outside the host) that make up the microbiota and the assembly of their collective genomes (the microbiome) [23,24,25]. Advances in in vitro culturing systems and methods have enabled the growth of single or collection of gut microbes outside of the host [26,27,28]. Germ-free animals (largely mice and rats), which are bred and raised free of all microbes, can be inoculated with a selected microbe or a collection of microbes to provide information on microbe-host interactions [29]. The use of these tools has advanced our understanding of the nature of the gut microbiota and its relationship with its hosts, as well as enabled the identification and characterization of new probiotic candidates and the nutrients that support or inhibit their growth. Recent studies have coupled these tools with genetic engineering of microbes (e.g., bacteria or yeast), demonstrating that some commensal microbes can be genetically customized and returned to the host where they impart new functions to the gut microbial community for the benefit of the host. These recent developments provide insights on potential ways to leverage commensal microbes (i.e., probiotics), specific nutrient formulations (prebiotics) or the combination of both (synbiotics) to optimize nutrition, provide therapy for certain metabolic conditions or avert onset of certain diseases in otherwise healthy people.

3. Relevance of Gut Microbiota to Human Health

A diverse gut microbiota is important for host health and maintenance of this community can be promoted by the consumption of foods that provide the large constellation of nutrients to support the growth of a wide variety of commensal microbial species [30]. The concerted effort of gut microbes is responsible for the processing of food components which yields new substrates for some members of the community through cross-feeding and also influences nutrient assimilation by the host (Figure 1)[31]. The importance of nutrient formulation in promoting the growth of specific microbes is exemplified by in vitro studies where pioneering researchers successfully cultured single species of bacteria, notably conventional probiotic lactobacilli, using a growth medium containing nutrients thought to be sufficient and easily metabolized [32]. However, it turns out that it is exceedingly difficult to culture the majority of resident gut microbiota in vitro primarily because the unique nutrient requirements of many of its members are not known [27,32]. Moreover, the physical environment (e.g., substratum, pH, atmosphere) and other factors that impact the growth of bacteria in vivo are difficult to replicate in vitro. Indeed, even the different segments of the mammalian host gut are inhabited by characteristic subsets of microbes that make up the entire gut microbiota [27]. Moreover, greater gut microbial diversity is characteristic of individuals that consume diets with high nutrient diversity and low energy density, and these individuals typically present a healthy metabolic profile [1,33]. On the other hand, suboptimal nutrition (low nutrient diversity and high energy density) promotes a dysbiotic bacterial profile (reduced diversity and a high Firmicutes to Bacteroidetes ratio) and contributes to a variety of host pathologies [33,34]. Early evidence of this strong influence of gut microbiota on host metabolism emerged in a study showing that transplantation of cecal microbiota from obese or lean mice to the gut of germ-free mice reproduces the obese or lean phenotype of the donor in the recipient animals [35]. A subsequent study recapitulated the findings using fecal microbiota from human twin donors discordant for the obesity phenotype; this study also demonstrated that the microbiota-induced obesity phenotype can be attenuated by feeding recipient animals with a low-fat high-fiber diet [36]. Gut microbiota-induced phenotypes have since been observed for other metabolic diseases including diabetes and cardiovascular disease as well as neurodegenerative diseases and cancers [37,38,39,40]. Given the apparent causal effect of gut microbiota composition on host health status, it is favorable to maintain a highly diverse population of commensal gut microbiota to preserve its beneficial contributions to the nutrition and health of the host.

Click to view original image

Figure 1 Interaction of the gut microbiota with its host. Dietary components and host factors influence the composition and diversity of the gut microbiota. Microbial proteins and microbe-produced metabolites provide substrates and signalling molecules that impact both the gut microbial community and its host. Some host factors modulate the growth and metabolic capacity of the gut microbial community. Administration of exogenous commensal microbes, as single species or as a collection of species, can augment the metabolic capacity of the resident gut microbial community. Alternatively, administration of engineered microbes without or with special prebiotics can reconstitute missing functions.

Pathogenic microbes naturally comprise a very small part of the gut microbial community and are normally outcompeted by the much greater abundance of commensal beneficial microbes [1,5]. Various factors can induce dysbiosis and the expansion of pathogenic microbes which may cause infection and other pathologies in the host. For example, use of antibiotics generally inhibits the growth of microbes, collaterally including commensal species, which creates a niche for antibiotic-resistant pathogenic microbes such as virulent strains of Clostridioides (formerly Clostridium) difficile to thrive [41]. It has been suggested that a virulent strain of C. difficile arose because it was unintentionally selected for by an ingredient used in the formulation of prepared food products [42]. Toxins produced by this bacterium act on intestinal cells and trigger host responses that lead to intestinal cell death and tissue injury [43]. Fortunately, oral administration of conventional probiotics, such as Lactobacillus rhamnosus GG, Bifidobacterium lactis BB-12 and selected mixtures along with the prescribed antibiotics has been shown to be effective in reducing the overall damage accrued during C. difficile infection giving the dysbiotic gut microbiota a chance to repair itself [44,45]. Another approach that has been shown to be highly effective in restoring heavily damaged gut microbiota is the transplantation of microbes from the stool of healthy human donors to individuals suffering from C. difficile infection [46,47]. This demonstrates the importance of a diversity of commensal microbes in promoting host health as well as reprogramming of dysbiosis.

The contributions of single commensal species within the gut microbial community to metabolic diseases are apparent from comprehensive studies assessing gut microbiota profiles of individuals at different disease states [37,48]. Follow-up studies to determine both gut microbiota and host responses to different therapeutic interventions highlight the combined efficacy of probiotic and dietary interventions in eliciting improvements in host metabolic status [49,50]. One prominent microbe of interest is Akkermansia muciniphila, a bacterial species that is inversely associated with gut barrier dysfunction, enhanced adiposity and glucose intolerance [51,52]. Supplementation with this microbe has been shown to improve gut barrier integrity, reduce adiposity and enhance insulin sensitivity not only in mice but also in humans [53,54,55]. Interestingly, administration of heat-killed preparation of A. muciniphila or its isolated Amuc_1100 protein can reproduce the observed therapeutic effect on the host, indicating that a specific protein encoded by this bacterium was responsible, at least in part, for the beneficial outcome [55]. This also exemplifies the potential of inactivated probiotics, their cellular components or products of their metabolic activity (collectively referred to as postbiotics) to contribute to beneficial health outcomes [56]. Other examples of prominent commensal bacteria include Faecalibacterium prausnitzii which is inversely associated with type 2 diabetes, as well as Parabacteroides distasonis which is inversely associated with obesity, in humans [49,57,58]. In these studies, the administration of live cultures of the respective microbe reduced the severity of metabolic disease and thus demonstrates that purposeful manipulation of the gut microbiota can contribute to the recovery of metabolic functions. Together, these findings highlight the crucial role that commensal species can have on host metabolism, reveal new probiotic candidates and how they can potentially be used in the design of probiotic-based therapies.

4. Gut Microbes Generate Products that Regulate Host Function

The diet is the major source of substrates that can be utilized by gut microbes, making it a critical determinant of the composition and function of the gut microbial community which, in turn, contributes to host metabolism (Figure 1). Amid the bioactive metabolites produced from gut microbial activity, short-chain fatty acids (SCFAs), including acetate, butyrate and propionate, are among the most abundant [59,60]. These SFCAs are produced in the gut predominantly through microbial fermentation of undigested dietary fibre but may also be generated from catabolism of amino acids [59,60]. Butyrate is the main energy source for intestinal cells and plays an important role in the stimulation of immune cell differentiation and maturation [61]. Interaction of SCFAs with free fatty acid receptors (members of the G protein-coupled receptor family) on enteroendocrine cells control release of gut peptide hormones (glucagon-like peptide 1, peptide YY) involved in appetite regulation and glucose homeostasis [62]. Furthermore, downstream effects of SCFA signalling such as the activation of the transcription factor peroxisome proliferator-activated receptor γ, can induce transcription of genes involved in fat oxidation in adipose tissue [63,64]. Studies also show that SCFAs can influence gene expression through epigenetic modifications by inhibiting histone deacetylases [64,65,66] which illuminates another strategy through which microbially-produced metabolites regulate host cellular functions. Intriguingly, these functions can be modulated by altering the dietary substrates that are accessible to gut microbiota [67,68,69], and illustrates how the tight interplay between the nutrient composition of the diet and gut microbiota metabolism regulates nutrient and energy metabolism of the host.

Gut microbiota can also transform dietary components into pathogenic metabolites. One example is the gut microbiota-initiated conversion of choline, phosphatidylcholine, betaine or L-carnitine, which are rich in animal-derived foods such as eggs, milk, red meat and seafood, into the pro-atherogenic metabolite trimethylamine N-oxide (TMAO) [70]. The process starts with microbial enzymatic conversion of dietary substrates to the gas, trimethylamine (TMA) which is then easily absorbed by the host and transported in blood to tissues including the liver where it is oxidized to TMAO by the hepatic enzyme flavin-containing monooxygenase 3 [71]. TMAO is thought to promote atherosclerosis by reducing reverse cholesterol transport and encourage macrophage recruitment to atherosclerotic plaques [72]. A high concentration of TMAO in human circulation is correlated with the development of cardiovascular disease [73,74]. A recent study showed that TMAO can bind to protein kinase R-like endoplasmic reticulum kinase or PERK [75] (an important intracellular protein involved in a cellular stress coping mechanism [76]), causing the induction of endoplasmic reticulum stress which is thought to be an integral part of the pathogenesis of many diseases [76,77,78]. Fortunately, our expanding understanding of the mechanisms through which microbially-produced metabolites contribute to human pathology allows the development of mitigation strategies such as the targeted inhibition of the microbial enzymes required for metabolite production and dietary modifications designed to reduce the production of harmful substrates.

Some host produced metabolites that can influence the composition of the gut microbiota are also substrates for microbial metabolism. One example is mucin, a host-produced glycoprotein that forms a protective barrier for the gut epithelium but also serves as an energy source for mucin-degrading species like Akkermansia muciniphila [79]. Bile acids are another class of host-derived metabolites that can either promote or inhibit the growth of gut microbes depending on the metabolic potential of the species. Bile acid metabolizing bacteria (e.g., Lactobacillus spp., Bacteroides spp.) secrete enzymes that can convert primary bile acids (produced in the liver) into secondary bile acids (products of microbial metabolism of primary bile acids) [80]. Notably, in humans, microbial enzymes catalyze the deconjugation and epimerization of the primary bile acid chenodeoxycholic acid to ursodeoxycholic acid (UDCA) which, on one hand, benefits the microbial community, and on the other hand, also has beneficial properties for the host [81]. Since UDCA is more hydrophilic than its precursor, it is less toxic to bacteria. Conversely, UDCA is a signalling molecule sensed by the host through the cell surface G-protein-coupled bile acid receptor TGR5 and nuclear receptor farnesoid X receptor, both involved in regulating glucose and energy homeostasis [82,83]. Additionally, recent studies demonstrate that UDCA is a proteostasis promoter that can alleviate endoplasmic reticulum stress [84,85]. This demonstrates that host-derived metabolites also offer new avenues for targeted modulation of the gut microbial composition to program the microbial production of metabolites that benefit the host.

5. Opportunities to Leverage Commensal Microbes in Nutrition Care Delivery

The intentional use of live commensal microbes for human health purposes has a long history of safety and acceptance which has recently been acknowledged in some modern nutrition therapies, particularly for gastrointestinal disorders [86,87]. For example, in the traditional management of lactose intolerance, a condition caused by gut bacterial fermentation of undigested, unabsorbed lactose, the diet is tailored to restrict or eliminate intake of lactose in order to circumvent the gastrointestinal discomfort caused by bacterially-produced gas and the osmotic diarrhea caused by the presence of lactose in the lumen [88,89]. It is also recommended to opt for fermented dairy products such as some yogurts that contain lactose-metabolizing bacteria and aged cheeses which have low lactose content [89]. Recent analyses recognize conventional probiotic species of the Lactobacillus and Bifidobacterium genera for the feature of lactase activity, emphasize advantages of supplementation in lactose intolerant individuals, and suggest potential use for improving the efficacy of the traditional nutritional management of lactose intolerance [90,91]. Other organisms currently accepted and used as probiotics include select strains of Saccharomyces and non-pathogenic strains of Escherichia coli typically used in treatment of diarrhea [87,92,93]. Although studies continue to evaluate the safety and therapeutic efficacy of existing probiotics, promising new organisms have emerged bearing probiotic features that can be used in disease-oriented nutrition-based interventions.

Personalized diet is an essential principle in the provision of modern nutrition care and considers health status, nutritional needs, food preferences and other influences on the efficacy of recommendations. Given the influence of gut microbiota on individual response to interventions, an important clinical question is whether the efficacy of dietary interventions can be optimized by accounting for individual gut microbial profiles in addition to host genetic composition. Intriguingly, a recent clinical trial compared the efficacy of a personalized diet with the Mediterranean diet in controlling postprandial glucose response in prediabetic individuals. Researchers demonstrated that dietary recommendations based on personalized predictions (including information about participant gut microbiota composition) led to greater improvement in glycemic control compared to the standard Mediterranean diet [94]. This suggests potential advantages of tailoring dietary recommendations based on physiological and gut microbiota characteristics. Although many challenges lie ahead in testing, optimizing, and standardizing the use of microbiota inclusive of dietary strategies in clinical practice, there are exciting opportunities for use in predicting response to nutrition intervention and personalized diet.

Behavioural changes are critical to the success of therapeutic interventions for metabolic diseases such as obesity, cardiovascular disease and diabetes. This includes adjustments to the nutrient quality of foods consumed and physical activity level. Recent evidence indicates that in addition to the effect of gut microbiota on host hunger and satiety, which affects the amount of food consumed, the gut microbiota could also influence host appetite for foods rich in specific nutrients [95,96]. Conversely, a recent study found an association between exercise intervention and gut microbiota fermentation in prediabetic individuals [97] while another study found that high abundance of some bacterial species, such as Veillonella spp., is associated with improved physical performance [98], suggesting that microbes contribute to mechanisms that determine host capacity for physical activity. These findings raise the question of whether specific organisms can be prudently leveraged to nudge host behaviours encompassed in food intake and physical performance.

The available technological capabilities for the isolation, in vitro culture and genetic programming of commensal microbes presents additional opportunities to refine the metabolic capacity of the microbiota itself (Table 1). In the case of phenylketonuria (PKU), affected individuals lack functional phenylalanine hydrolase and are highly susceptible to adverse neurological outcomes due to toxicity caused by accumulation of phenylalanine in the blood. Hence, the standard treatment of individuals with PKU features a diet restricted in phenylalanine and supplemented with tyrosine. Intriguingly, a group of researchers recently engineered a strain of Escherichia coli Nissle 1917, called SYNB1618, to metabolize phenylalanine in the lumen of the gut [99] and subsequently demonstrated the clinical safety and efficacy of this organism in humans [100] thereby showing how specific microbes can provide functions originally missing in the host. Other studies have demonstrated the programming of commensal microbes for surveillance of specific pathogens [101], removal of pathogenic metabolites [102,103] and reinforcement of natural host metabolic functions [104,105]. As well, prokaryotic viruses (e.g., bacteriophages) may be useful in specifically targeting certain species of bacteria for genetic modification (e.g., as stable prophages) or eradication [106,107]. The use of these strategies for therapies will likely be in the form of synbiotics whereby microbes are administered along with the nutrients required for their growth in the host (Figure 1). Overall, cautious and targeted application of engineered probiotics presents an exciting new avenue for the treatment of some genetic and acquired metabolic diseases.

Table 1 Examples of conventional, candidate probiotics and engineered commensal microbes.

6. Summary

Humans have co-evolved complex relationships with microbes and have historically benefitted from the consumption of microbes in fermented foods. Progression in our understanding of the gut microbiota-host relationship has revealed different ways that microbes may confer benefit or cause harm to its host. Recent progress suggests it is not only possible to shape the composition and metabolic capacity of the gut microbiota to a more desirable profile with probiotics and prebiotics, but also impart special functions using engineered commensal microbes. Newly identified beneficial and engineered microbes indicate promising avenues for improving nutrition and developing nutrition-based therapies for metabolic disorders. Even though single species that appear to be therapeutic have been identified, the most effective strategy to restore gut microbial health is likely a collection of probiotic microbes together with the nutrients they require to thrive in the gut. Equipped with knowledge about gut microbiota composition and metabolic potential, it is possible to design diets or specific nutrition interventions that either supply the nutrients (prebiotics) required to alter the composition of the gut microbiota or supplement individuals with specific probiotics or even postbiotics to impart a desired health benefit.

Author Contributions

AMS and LBA conceived, wrote, and edited the manuscript.

Funding

Research in our laboratory is funded by grants from the Natural Sciences and Engineering Research Council of Canada and the Canadian Institutes of Health Research.

Competing Interests

The authors have declared that no competing interests exist.

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