OBM Neurobiology is an international peer-reviewed Open Access journal published quarterly online by LIDSEN Publishing Inc. By design, the scope of OBM Neurobiology is broad, so as to reflect the multidisciplinary nature of the field of Neurobiology that interfaces biology with the fundamental and clinical neurosciences. As such, OBM Neurobiology embraces rigorous multidisciplinary investigations into the form and function of neurons and glia that make up the nervous system, either individually or in ensemble, in health or disease. OBM Neurobiology welcomes original contributions that employ a combination of molecular, cellular, systems and behavioral approaches to report novel neuroanatomical, neuropharmacological, neurophysiological and neurobehavioral findings related to the following aspects of the nervous system: Signal Transduction and Neurotransmission; Neural Circuits and Systems Neurobiology; Nervous System Development and Aging; Neurobiology of Nervous System Diseases (e.g., Developmental Brain Disorders; Neurodegenerative Disorders).

OBM Neurobiology  publishes a variety of article types (Original Research, Review, Communication, Opinion, Comment, Conference Report, Technical Note, Book Review, etc.). Although the OBM Neurobiology Editorial Board encourages authors to be succinct, there is no restriction on the length of the papers. Authors should present their results in as much detail as possible, as reviewers are encouraged to emphasize scientific rigor and reproducibility.

Publication Speed (median values for papers published in 2023): Submission to First Decision: 7.5 weeks; Submission to Acceptance: 15.9 weeks; Acceptance to Publication: 7 days (1-2 days of FREE language polishing included)

Current Issue: 2024  Archive: 2023 2022 2021 2020 2019 2018 2017
Open Access Original Research

Possible Preventive Effect of Ziziphora clinopodioides Lam. Essential Oil on Some Neurodegenerative Disorders

Naira Sahakyan 1,2,*, Margarit Petrosyan 1

  1. Department of Biochemistry, Microbiology & Biotechnology, Yerevan State University, 1A. Manoogian Str., 0025 Yerevan, Armenia

  2. Research Institute of Biology, Yerevan State University, 1A. Manoogian Str., 0025 Yerevan, Armenia

Correspondence: Naira Sahakyan

Academic Editor: Tomohiro Chiba

Received: June 10, 2022 | Accepted: September 27, 2022 | Published: October 08, 2022

OBM Neurobiology 2022, Volume 6, Issue 4, doi:10.21926/obm.neurobiol.2204140

Recommended citation: Sahakyan N, Petrosyan M. Possible Preventive Effect of Ziziphora clinopodioides Lam. Essential Oil on Some Neurodegenerative Disorders. OBM Neurobiology 2022; 6(4): 140; doi:10.21926/obm.neurobiol.2204140.

© 2022 by the authors. This is an open access article distributed under the conditions of the Creative Commons by Attribution License, which permits unrestricted use, distribution, and reproduction in any medium or format, provided the original work is correctly cited.


The present article describes some characteristics of the effect of essential oil (EO) extracted from Ziziphora clinopodioides harvested from Armenian highlands on microglial cell lines (BV-2 wild-type (WT) and acyl-CoA oxidase1 (ACOX1)-deficient (Acox1–/–) cells). The mutant cell line was used as a model to investigate cellular oxidative damage following EO treatment. The main components of the tested EO were pulegone, isomenthone, 1,8-cineole, piperitone, and neomenthole, with concentrations of 42.1%, 9.7%, 8.22%, 7.35%, and 5.9%, respectively, in plants harvested from the high-altitude Armenian landscape. The IC50 value of the EO in the DPPH assay was 7.025 µL/mL. The sub-cytotoxic concentrations (based on the MTT assay) for both cell lines were 5 × 10–1 µL/mL. The catalase activity of the WT cells was decreased following 24-h treatment with the EO, but that of Acox1–/ BV-2 cellswas increased. ACOX1 activity was decreased (up to 49%) at 72hof treatment. These results show the protective effect of the tested EO on Acox1–/– mutantcells.


Ziziphora; pulegone; microglia; catalase; acetyl-CoA oxidase 1; cytotoxicity

1. Introduction

Organic substances produced by plants are structurally and functionally diverse metabolites that are not only important for the adaptation and survival of plants but are also beneficial to humans in several aspects. These primary and secondary metabolites play many different roles in plant growth and development and in response to external factors [1,2,3]. Plants of the Lamiaceae family are the most diverse and widespread in terms of ethnomedicine and are among the most thoroughly studied ones. These plants possess a broad spectrum of biological activity, including antibacterial and antioxidant activities [4,5,6,7]. Ziziphora clinopodioides Lam. is a widely distributed perennial aromatic plant belonging to this family. It has been used in folk medicine since ancient times [8]. The medicinal value of this plant is mainly based on the yield of essential oil (EO) and its chemical composition [8,9]. The main components of this plant EO are thymol, pulegone, menthone, isomenthone, 1,8-cineole, and piperitone. According to the literature, all these metabolites possess antibacterial and antioxidant activities [4,9,10]. Benabdallah et al. (2018) showed the acetylcholinesterase inhibitory activity of pilegone, one of the main compounds of the investigated EO [11]. Sedighi et al. (2019) reported the neuroprotective effect of Z. clinopodioides hydroalcoholic extracts on a rat model of Alzheimer’s disease [12]. Other studies have also provided evidence of the neuroprotective effect of different extracts of this plant and their individual components based on their antioxidant activity [13,14].

On the basis of the abovementioned findings, we aimed to investigate the effect of the Z. clinopodioides EO components on the activity of antioxidant enzymes of the specialized central nervous system macrophages, namely microglial cells, which play a role in brain development regulation. For this purpose, the following two neuronal microglial cell lines were used: BV-2 wild-type (WT) cells and acyl-CoA oxidase 1 (ACOX1)-deficient (Acox1–/–) cells [15,16]. The mutant cells show accumulation of very long-chain fatty acids (VLCFAs) and generation of proinflammatory cytokines, revealing their promising application as a model to investigate changes in peroxisomal β-oxidation on oxidative stress, inflammation, and cellular oxidative damage [17,18]. The selected cell models meet three essential criteria for their application: they are well studied, are easy to cultivate, and play a central role in the development of oxidative stress-induced changes.

2. Materials and Methods

2.1 Plant Material

Z. clinopodioides plants were collected during the flowering season (July 2017–2019, Kotayk region, 1500–1600 m a.s.l.). The plants were identified at the Institute of Botany, National Academy of Sciences of Armenia, Yerevan, Armenia, and a voucher specimen number was provided. Plant samples are included in the herbarium of the same department and are available upon request.

2.2 Extraction of EO

EOs were extracted from air-dried plant material (aerial parts alone) by hydro distillation using a Clevenger-type apparatus as described previously [19].

2.3 Determination of Radical Scavenging Activity

Free radical scavenging activity of the tested EO was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay [20]. Catechin was used as a positive reference. The sample solution contained 125 µL (1 mM) DPPH, 375 µL ethanol, and 500 µL of test solution (EOs or catechin at different concentrations). In the control solution, the test solution was replaced with ethanol. The absorbance was measured at 514 nm [21].

2.4 Determination of EO Chemical Composition

Gas chromatography (GC)-mass spectrometry (MS) analysis of the EOs was performed using a Hewlett-Packard 5890 Series II gas chromatograph as described previously [21,22].

2.5 Cell Cultures

The test cell lines (BV-2, Acox1–/mutants and WT cells) were provided by the Laboratory BioPeroxIL: Laboratoire de Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique, Université de Bourgogne, Dijon, France.

2.6 BV-2 Microglia Cell Culture

Murine microglial BV-2 cell lines (BV-2, Acox1–/mutants and WT cells) were cultivated as described previously [23].

2.7 MTTAssay

Cell proliferation (mitochondrial activity) was measured by 3-(4,5-dimethyltrazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cells were seeded onto 96-well plates and then treated with various concentrations of Z. clinopodioides EO (50 to 5 × 10–4 µL/mL) for 24–72 h [23]. This test was performed to clarify the effect of the EOnon microglial cell viability.

2.8 BV-2 Cell Lysate Preparation

Following the treatment of BV-2 microglial cells with the EO, the cells were washed with phosphate-buffered saline (PBS) and lysed by the addition of 50 µL of radioimmunoprecipitation (RIPA) buffer as described previously [23]. The protein content was measured according to the method of Smith et al.(1985) [24].

2.9 Enzymatic Activity Measurement

Catalase and ACOX1 activities in fresh cell lysate were measured according to the method of Cherkaoui-Malki et al. [25] and Oaxaca-Castillo D. et al. (2007) [26]. Data are expressed as units/mg of protein.

2.10 Data Processing

Statistical analysis was conducted using Student’s t-test (Excel software), and the differences were considered statistically significant at p< 0.05.

3. Results

3.1 Chemical Composition and Antioxidant Activity of Z. clinopodioides EO

The average yield of the EO was approximately1%. The EO of Z. clinopodioides harvested from the high-altitude Armenian landscape was rich in pulegone (42.1%), isomenthone (9.7%), 1,8-cineole (8.22%), piperitone (7.35%), and neomenthole (5.9%) (Figure 1).

Click to view original image

Figure 1 Chemical composition of Z. clinopodioides essential oil.

Our experiments confirmed the scientific literature data that the EO of Z. clinopodioides shows high antioxidant activity in chemical-based tests; it showed low IC50 value (7.025 ±0.9 µL/mL) ((R2 = 0.851) (data not shown)) as expected (IC50 value of the positive control catechin was 12.62 ±0.8 µg/mL) ((R2 = 0.899) (data not shown)), which indicates its high potential in scavenging free radicals [19,27].

3.2 Effect of Z. clinopodioides EO on BV2 Cell Viability (MTT Assay)

The sub-cytotoxic concentration of EO for both cell lines was determined as 5 × 10−1 µL/mL (Figures 2A, 2B) (p< 0.05). This concentration was used in further analyses. This concentration also allowed to avoid the toxic effect of the EO and to determine only its activity on antioxidant enzymes.

Click to view original image

Figure 2 Effects of Z. clinopodioides EO on the viability of BV-2 WT and Acox1–/microglial cells (MTT assay; A and B, respectively). Cells were treated for 24 h with the EO at various concentrations (50 to 5 × 10–4 µL/mL). Results are expressed as mean ±SD of three repetitions (p< 0.05 for both BV-2 cell lines).

Cell viability of WT cells increased following treatment with the EO for 24 and 48h, while the cell viability of the mutant cells increased only after 24hof treatment (Figure 3). No statistically significant changes were observed for other cases (p ˃ 0.05).

Click to view original image

Figure 3 Influence of the Z. clinopodioides EO on the cell viability of BV-2 WT and Acox1–/microglial cells (MTT assay; A and B, respectively). Cells were treated with the EO at 5 × 10−1 µL/mL concentration. Results are expressed as mean ±SD of three repetitions (* indicates p< 0.05, ** indicates p ˃ 0.05, for both cell lines).

3.3 Effect of Z. clinopodioides EO on Peroxisomal Functions in BV-2 Cells

BV-2 WT cells showed a decrease in catalase activity at 24 h of treatment with EO, while Acox1–/– BV-2 cells showed an increase in catalase activity after treatment. Although the activity of this enzyme was increased in both cell lines at 72hof treatment with the EO, the difference was not statistically significant (p ˃ 0.05) (Figure 4).

Click to view original image

Figure 4 Effect of the EO on the catalase activity of BV-2 WT and mutant microglial cells (A and B, respectively). *p < 0.05; **p ˃ 0.05. Cells were treated with the EO at 5 × 10−1 µL/mL concentration.

The Z. clinopodioides EO caused a considerable decrease in ACOX1 activity (up to 40%) at 72hof treatment (Figure 5) (p< 0.05). We also observed an increase in the catalase activity of BV-2 WT cells (up to 20%) only after 48h of treatment (p ˃ 0.05).

Click to view original image

Figure 5 Effect of Z. clinopodioides EO on palmitoyl-CoA oxidase type 1 activity of BV-2 WT cells. Cells were treated with the EO at 5 × 10–1 µL/mL concentration. Results are expressed as mean ±SD of 3 repetitions (*p < 0.05; **p ˃ 0.05).

4. Discussion

Z. clinopodioides EO is rich in pulegone, isomenthone, piperitone, and 1,8-cineole [8]. As reported previously [5], our present study results confirmed these findings data and revealed that the chemical composition of the EO of Z. clinopodioides from high-altitude Armenian landscape was very similar to those described in literature. This implies that despite the differences between the elevation of the growing area of Z. clinopodioides and climatic conditions, this plant produces approximately the same components of EO [5,8,19]. In contrast to the literature data [9,10], wherein thymol was reported to be one of the main components in the EO extracted from Z. clinopodioides of Armenian flora, the concentration of this component in the present study did not exceed 0.35%. All these components show reducing capability [28], which implies that they can alter the activity of different enzymes [29].

Ahmadi et al. (2021) [8] reported that the Z. clinopodioides EO shows a high cytotoxic effect on human lymphocytes at 1–10 µL/mL concentration. As mentioned earlier, we used a lower concentration of the EO in our experiments, which did not have a significant effect on the viability of the tested cell lines but could exert antioxidant activity in chemical-based assays.

The evaluation of the activity of ACOX1 – the rate-limiting enzyme involved in peroxisomal β-oxidation– and of the activity of catalase – the main peroxisomal antioxidant enzyme in the tested microglial cells– after treatment with Z. clinopodioides EO is of interest as these enzymes play a crucial role in maintaining redox balance of cells [30]. Peroxisomes also contain enzymes that catalyze the production of hydrogen peroxide (H2O2), superoxide (O2), or nitric oxide (NO) as part of their normal catalytic cycle. These molecules react with other molecules to form other reactive oxygen species and reactive nitrogen species [23]. Some of the main antioxidant enzymes, such as catalase and superoxide dismutase, play critical and sometimes even a central role in the cellular antioxidant defense process by catalyzing the dismutation and subsequent quenching of H2O2, including those, which form from the enzymatic oxidation by the action of ACOX1 [15].

Our present study results showed that the tested concentration of Z. clinopodioides EO influenced the activity of the main cellular antioxidant enzymes catalase and ACOX1. Catalase activity was decreased in WT cells, which indicated that the treatment did not induce stress in these cells. Catalase activity was increased in ACOX1-deficient cells at 24hof treatment. This tendency was, however, not retained with the increase in EO treatment duration. This phenomenon can serve as an indicator of the protective effect of the Z. clinopodioides EO components on mutant cells [27,29].

5. Conclusion

In summary, Z. clinopodioides EO shows remarkable antioxidant properties in chemical-based assays, and it can also alter the activity of cellular antioxidant enzymes such as catalase and ACOX1. The findings of the present study confirm that the EO extracted from Z. clinopodioides harvested from the high-altitude Armenian landscape affects the cellular antioxidant activity of microglial cells and can prevent neurodegenerative changes such as the accumulation of VLCFA in cells. Because of this favorable property, the tested Z. clinopodioides EO can be used in preventive medicine.

List of Abbreviations


acyl-CoA oxidase type 1


Dulbecco’s modified Eagle medium




ethylenediamine tetraacetic acid


essential oil


fetal bovine serum


chromatography mass spectrometry


3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide


National Institute of Standards and Technology


Phosphate-buffered saline 




Superoxide dismutase


very-long-chain fatty acids


wild type


This work is funded by the Science Committee of RA, in the frames of the research project № 21AG-4D027 as well as by Basic support from Committee of Science, Ministry of Education, Science, Culture and Sport of Armenia. This article is also based upon work from COST Action NutRedO CA 16112 supported by COST (European Cooperation in Science and Technology). Special gratitude to Prof. Mustapha Cherkaoui-Malki, Dr. Pierre Andreoletti for formation of this article as well as “NAIRIAN” CJSC for providing investigation material.

Author Contributions

Conceptualization, N.S. and M.P.; methodology, N.S.; investigation, N.S.; data curation, N.S., N.S..; writing—review and editing, N.S and M.P; supervision, N.S. All authors have read and agreed to the published version of the manuscript.

Competing Interests

The authors have declared that no competing interests exist.


  1. Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, et al. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv. 2015; 33: 1582-1614. [CrossRef]
  2. Trchounian A, Petrosyan M, Sahakyan N. Plant cell redox homeostasis and reactive oxygen species. In: Redox state as a central regulator of plant-cell stress responses. Cham: Springer; 2016. pp.25-50. [CrossRef]
  3. Wang S, Alseekh S, Fernie AR, Luo J. The structure and function of major plant metabolite modifications. Mol Plant. 2019; 12: 899-919. [CrossRef]
  4. Sahakyan N, Petrosyan M, Koss-Mikołajczyk I, Bartoszek A, Sad TG, Nasim MJ, et al. The Caucasian flora: A still-to-be-discovered rich source of antioxidants. Free Radic Res. 2019; 53: 1153-1162. [CrossRef]
  5. Sahakyan NZh, Petrosyan MT, Trchounian AH. Some lamiaceae family plant essential oil chemical composition and their potential as antimicrobial agents against antibiotic-resistant bacteria. Proc Yerevan State Univ. 2019; 53: 23-28.
  6. Mamadalieva NZ, AkramovDKh, Böhmdorfer S, Azimova SS, Rosenau T. Extractives and biological activities of lamiaceae species growing in Uzbekistan. Holzforschung. 2020; 74: 96-115. [CrossRef]
  7. Ramos da Silva LR, Ferreira OO, Cruz JN, de Jesus Pereira Franco C, Oliveira dos Anjos T, Cascaes MM, et al. Lamiaceae essential oils, phytochemical profile, antioxidant, and biological activities. Evid Based Complement Altern Med. 2021. doi:10.1155/2021/6748052. [CrossRef]
  8. Ahmadi A, Gandomi H, Derakhshandeh A, Misaghi A, Noori N. Phytochemical composition and in vitro safety evaluation of Ziziphora clinopodioides lam. Ethanolic extract: Cytotoxicity, genotoxicity and mutagenicity assessment. J Ethnopharmacol. 2021; 266: 113428. [CrossRef]
  9. Hazrati S, Govahi M, Sedaghat M, Kashkooli AB. A comparative study of essential oil profile, antibacterial and antioxidant activities of two cultivated Ziziphora species (Z. clinopodioides and Z. tenuior). Ind Crops Prod. 2020; 157: 112942. [CrossRef]
  10. Shahbazi Y. Chemical compositions, antioxidant and antimicrobial properties of Ziziphoraclinopodioides lam. Essential oils collected from different parts of Iran. J Food Sci Technol. 2017; 54: 3491-3503. [CrossRef]
  11. Benabdallah A, Boumendjel M, Aissi O, Rahmoune C, Boussaid M, Messaoud C. Chemical composition, antioxidant activity and acetylcholinesterase inhibitory of wild Mentha species from northeastern Algeria. S Afr J Bot. 2018; 116: 131-139. [CrossRef]
  12. Sedighi S, Tehranipour M, Vaezi G, Hojati V, Hashemi-Moghaddam H. The effect of hydroalcoholic extract of Ziziphora clinopodioides L. On spatial memory and neuronal density of hippocampal CA1 region in rats with sporadic Alzheimer’s disease. Avicenna J Phytomed. 2019; 9: 362-373.
  13. Abdolsamad Halaf IA, Tehranipour M, Mahmodzadeh Akharat H. Effect of aqueous and alcoholic extracts of Ziziphora clinopodioides on apoptosis and alteration of caspase-3 and caspase-9 gene expression in anterior horn neurons of the spinal cord after sciatic nerve compression in male rats. Yektaweb. 2021; 28: 222-235. [CrossRef]
  14. Crupi R, Impellizzeri D, Bruschetta G, Cordaro M, Paterniti I, Siracusa R, et al. Co-ultramicronized palmitoyl ethanolamide/luteolin promotes neuronal regeneration after spinal cord injury. Front pharmacol. 2016; 7: 47. [CrossRef]
  15. Raas Q, Saih FE, Gondcaille C, Trompier D, Hamon Y, Leoni V, et al. A microglial cell model for acyl-CoA oxidase 1 deficiency. BiochimBiophys Acta Mol Cell Biol Lipids. 2019; 1864: 567-576. [CrossRef]
  16. Vluggens A, Andreoletti P, Viswakarma N, Jia Y, Matsumoto K, Kulik W, et al. Functional significance of the two ACOX1 isoforms and their crosstalks with PPARα and RXRα. Lab Investig. 2010; 90: 696-708. [CrossRef]
  17. Vamecq J, Andreoletti P, El Kebbaj R, Saih FE, Latruffe N, El Kebbaj M, et al. Peroxisomal acyl-CoA oxidase type 1: Anti-inflammatory and anti-aging properties with a special emphasis on studies with LPS and argan oil as a model transposable to aging. Oxid Med Cell Longev. 2018; 2018.doi:10.1155/2018/6986984. [CrossRef]
  18. Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: Current state. Nutr J. 2016; 15:71. doi: 10.1186/s12937-016-0186-5. [CrossRef]
  19. Avetisyan A, Markosian A, Petrosyan M, Sahakyan N, Babayan A, Aloyan S, et al. Chemical composition and some biological activities of the essential oils from basil ocimum different cultivars. BMC Complement Altern Med. 2017; 17: 60. [CrossRef]
  20. Apak R, Gorinstein S, Böhm V, Schaich KM, Özyürek M, Güçlü K. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC technical report). Pure Appl Chem. 2013; 85: 957-998. [CrossRef]
  21. Moghrovyan A, Sahakyan N, Babayan A, Chichoyan N, Petrosyan M, Trchounian A. Essential oil and ethanol extract of oregano (Origanum vulgare L.) from Armenian flora as a natural source of terpenes, flavonoids and other phytochemicals with antiradical, antioxidant, metal chelating, tyrosinase inhibitory and antibacterial activity. CurrPharmDes. 2019; 25: 1809-1816. [CrossRef]
  22. Moghrovyan A, Parseghyan L, Sevoyan G, Darbinyan A, Sahakyan N, Gaboyan M, et al. Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide. Korean J Pain. 2022; 35: 140-151. [CrossRef]
  23. Sahakyan N, Andreoletti P, Petrosyan M, Cherkaoui-Malki M. Essential oils of basil cultivars selectively affect the activity of antioxidant enzymes in murine glial cells. 2022; 3. doi: 10.2174/2665978602666211217143112. [CrossRef]
  24. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985; 150։ 76-85. [CrossRef]
  25. Malki CM, Bardot O, Lhuguenot JC, Latruffe N. Expression of liver peroxisomal proteins as compared to other organelle marker enzymes in rats treated with hypolipidemic agents. Biol Cell. 1990; 69: 83-92. [CrossRef]
  26. Oaxaca-Castillo D, Andreoletti P, Vluggens A, Yu S, Van Veldhoven PP, Reddy JK, et al. Biochemical characterization of two functional human liver acyl-CoA oxidase isoforms 1a and 1b encoded by a single gene. BiochemBiophys Res Commun. 2007; 360: 314-319. [CrossRef]
  27. Sahakyan N, Andreoletti P, Cherkaoui-Malki M, Petrosyan M, Trchounian A. Artemisia dracunculus L. Essential oil phytochemical components trigger the activity of cellular antioxidant enzymes. J Food Biochem. 2021; 45: e13691. [CrossRef]
  28. Božović M, Ragno R. Calamintha nepeta (L.) savi and its main essential oil constituent pulegone: Biological activities and chemistry. Molecules. 2017; 22: 290. [CrossRef]
  29. Ginovyan M, Andreoletti P, Cherkaoui-Malki M, Sahakyan N. Hypericum alpestre extract affects the activity of the key antioxidant enzymes in microglial BV-2 cellular models. AIMS Biophysics. 2022; 9: 161-171. [CrossRef]
  30. Zeng J, Deng S, Wang Y, Li P, Tang L, Pang Y. Specific inhibition of acyl-CoA oxidase-1 by an acetylenic acid improves hepatic lipid and reactive oxygen species (ROS) metabolism in rats fed a high fat diet. J Biol Chem. 2017; 292: 3800-3809. [CrossRef]
Download PDF Download Citation
0 0