Cytoplasmic Microinjection of piggyBac Transposase mRNA and Transposon Vectors for Efficient In Vitro Production of Transgenic Porcine Parthenotes






Abstract
(ISSN 2577-5790)
OBM Genetics is an international Open Access journal published quarterly online by LIDSEN Publishing Inc. It accepts papers addressing basic and medical aspects of genetics and epigenetics and also ethical, legal and social issues. Coverage includes clinical, developmental, diagnostic, evolutionary, genomic, mitochondrial, molecular, oncological, population and reproductive aspects. It publishes research articles, reviews, communications and technical notes, etc. There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.
Archiving: full-text archived in CLOCKSS.
Rapid publication: manuscripts are undertaken in 15.0 days from acceptance to publication (median values for papers published in this journal in the second half of 2021, 1-2 days of FREE language polishing time is also included in this period).
Special Issue
Somatic Cell Genetics and Its Applications
Submission Deadline: September 30, 2022 (Open) Submit Now
Guest Editor
Ahmad Omar, PhD
Department of Horticultural Sciences, University of Florida, Citrus Research and Education Center, IFAS, Lake Alfred, USA
Research interests: citrus scion and rootstock improvement, tissue culture and genetic modifications, protoplast techniques, somatic cell genetics, somaclonal variation, embryo rescue, ploidy manipulation, disease resistance, transformation, propagation, molecular biology, metabolic, genome editing using CRISPR cas9 technology
Publication
Cytoplasmic Microinjection of piggyBac Transposase mRNA and Transposon Vectors for Efficient In Vitro Production of Transgenic Porcine Parthenotesby
![]() ![]() ![]() ![]() ![]() ![]() Abstract The efficient production of transgenic (Tg) piglets has remained a challenge in the field of domestic animal studies. Unlike mice, the pronuclei of pig zygotes cannot be easily studied because of the abundance of lipid droplets. Therefore, the zygotes must be briefly centrifuged before pronuclear injection (PNI) to move the lipid droplets [...] |
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