Journal Flyer

Current Issue 2018  Archive 2017 
Biography
Biography

Editor-in-Chief of OBM Hepatology and Gastroenterology

Osamu Yokosuka is an Emeritus Professor of Chiba University, Japan. He graduated from Chiba University School of Medicine in 1975 then worked as a trainee under Professor K. Okuda in Chiba University Hospital till 1978. Dr. Yokosuka was a research fellow worked under Professor S. Scherlock and Professor B. H. Billing in Royal Free Hospital, London, UK from 1978 to 1980; under Professor M. Omata in Chiba University from 1980 to 1985; and under Dr. J Summers in Fox Chase Cancer Center, PA, USA in 1984. In 1985, he received a Degree of Doctor of Medical Science, and served as an Assistant Professor in Chiba University till 1994, then as Lecturer in Medicine till 2006 when he was appointed as Director and Professor of Medicine. From 2013 to 2015, he served as the Dean of Chiba University School of Medicine.

Dr. Yokosuka was the Secretary General of APASL (2008-2014). In 2016, he was elected as the President of APASL Tokyo, the President of 52nd Annual Meeting of Japan Society of Hepatology, and the President of Funabashi Central Hospital. Dr. Yokosuka’s research mainly focuses on Hepatitis and Hepatocellular Carcinoma. So far, he has published more than 700 original papers.

Biography

The Associate Editor of OBM Hepatology and Gastroenterology

Tatsuo Kanda received a medical degree in 1991 at Niigata University School of Medicine, Japan, and his PhD in 1999 at Chiba University Graduate School of Medicine, Japan. He had post-doctor training for 3 years under Prof. Ratna Ray and Prof. Ranjit Ray at Saint Louis University, St. Louis, MO, USA. In Dec. 2008, Tatsuo Kanda became a Tenure-track Associate Professor at Department of Medicine and Clinical Oncology, Chiba University, Graduate School of Medicine, Japan. In Feb. 2013, Tatsuo Kanda was nominated a permanent Associate Professor at Department of Gastroenterology and Nephrology, Chiba University, Graduate School of Medicine, Japan. In 2017, Tatsuo Kanda became an Associate Professor, Division of Gastroenterology and Hepatology, Department of Internal Medicine, Nihon University School of Medicine. For ~25 years, he has focused his scientific interests on the topics related to liver diseases including acute liver failure, viral hepatitis and autoimmune liver diseases, and worked with Prof. Osamu Yokosuka. Tatsuo Kanda is also an expert for hepatitis A virus (HAV), HBV and HCV, and translation and replication of these viruses, and hepatocarcinogenesis. With his expertise in antiviral therapies and hepatitis virus research, Tatsuo Kanda also sees a lot of patients in clinical daily practice. Tatsuo Kanda has published more than 200 articles in peer-reviewed Journal.

Special Issue

Applications of Fluorescence in Situ Hybridization

Submission Deadline: July 31, 2018 (Open)                Submit Now

Guest Editor

Thomas Liehr, PhD, Dr.h.c., inv. Prof. (YSU and BMS)
University Clinic Jena, Institute of Human Genetics, Am Klinikum 1, 07747 Jena, Germany
E-Mail: [email protected]
Website: http://www.humangenetik.uniklinikum-jena.de/Forschung/Molekulare+Zytogenetik/Mitarbeiter.html
Research Interests: clinical genetics; (molecular) cytogenetics; interphase architecture; ZOO-FISH; marker and derivative chromosomes

About This Topic

Fluorescence in situ hybridization (FISH) is an approach applied and applicable in many fields of biological research and diagnostics. Its unique feature is to provide information on localization and copy numbers of specific stretches of nucleic acids on the single cell level. Thus, FISH cannot be replaced by, like in medical field at present very popular ‘high throughput approaches’, which are mostly providing information on the genetic content of thousands to millions of cells at a time. FISH provides multiple possibilities and variants, according to the question to be answered. Fluorescence in situ hybridization can be applied as (i) single to multi-color FISH approaches, (ii) using DNA, RNA, cDNA, PNA, and other types of labeled nucleotides as probes; also (iii) samples for FISH can be various human, animal or plant derived tissue types, and even single cellular organisms.

In this special issue of OBM Genetics, we have selected topics that highlight the progress, the state-of–the-art and future potential of fluorescence in situ hybridization, ranging from practical and technological aspects to applications in research and diagnostics of fluorescence in situ hybridization in human, animal, plant, fungi, biofilms and bacteria. Overall this issue provides information where and how fluorescence in situ hybridization can be applied in practice, and shows its high potential in diagnostics and research.

Planned Papers

Title: Easy approach to gain interphase cells for fluorescence in situ hybridization
Authors: Thomas Liehr*, Monika Ziegler
Affiliations: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany

Title:Murine multicolor banding in solid tumor cell lines
Authors: Thomas Liehr 1,*, Eva Kubicova 1, Lisa-Marie Barf 1, Hans Rhode 1, Ralf Steinacker 1, Lioba Hergenhahn 1, Mattia Manferrari 1,2, Karolina Guja 1,3, Nadezda Kosyakova 1, Martina Rincic 4 , Shaymaa S. Hussein Azawi 1.
Affiliations: 1. Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany;
2. University of Trieste, Department of Life Sciences, Trieste, Italy;
3. Department of Plant Anatomy and Cytology Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland;
4. Department for Functional Genomics, Centre for Translational and Clinical Research, University Hospital Centre Zagreb, University of Zagreb School of Medicine, Zagreb, Croatia

Title: Interphase Fluorescence in Situ Hybridization on products of conception
Authors: Iryna Tkach 1, Nataliya Huleyuk 1, Danuta Zastavna 1,2, Thomas Liehr 3, Ewa Ciszkowicz 2
Affiliations: 1. Institute of Hereditary Pathology NAMS of Ukraine, Lviv, Ukraine
2.Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology,Rzeszow, Poland
3.Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany

Title: Characteristics of prenatal diagnosis by FISH in Cuba
Authors: Luis Alberto Mendez-Rosado, Odalis Molina, Andurina Barrios
Affiliations: National Center of Medical Genetic of Cuba

Title: Molecular diagnosis (FISH) of patients with neurodevelopmental disorders
Authors: Luis A. Mendez-Rosado, Damaris Garcia, Odalis Molina, Anduriña Barrios
Affiliation: National Center of Medical Genetic of Cuba

Title: Identification of a supernumerary marker chromosome involving 11p14.1q22.1 region in a prenatal case: clinical and molecular characterization.
Authors: Kontodiou Maria 1, Paspaliaris Vassilis 1, Dagklis Themistoklis 2, Siomou Elisavet 1, Al-Rikabi AH 3, Tsita Kalliopi 4, Stavroulaki Theano 4, Pampanos Andreas 4, Papaioannou Georgios 5, Papoulidis Ioannis 1, Manolakos Emmanouil 1,6,7
Affiliations: 1. Access to Genome P.C., Clinical Laboratory Genetics, Athens-Thessaloniki, Greece;
2. 3rd Obstetrics and Gynecology clinic, Ippokrateion Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece;
3. Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany.
4. Alexandra General Hospital of Athens, Athens, Greece;
5. Department of Gynecology, Attikon Hospital, University of Athens, Athens, Greece;
6. Developmental assessment unit, 2nd department of pediatrics, P. & A. Kyriakou children's hospital, School of medicine, National and Kapodistrian University of Athens, Athens, Greece;
7. Department of Medical Genetics, University of Cagliari, Binaghi Hospital, Cagliari, Italy.

Title: FISHing for an unstable cellular genome in the human brain
Authors: Yuri B Yurov 1,2, Svetlana G Vorsanova 1,2, Ivan Y Iourov 1,2,3
Affiliations: 1. Mental Health Research Center, Moscow, Russia;
2. Russian National Research Medical University named after N.I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russia;
3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health, Moscow, Russia

Title: FISH-based approaches for detecting mosaic aneuploidy
Authors: Svetlana G Vorsanova 1,2, Yuri B Yurov 1,2, Ilia V Soloviev 2, Alexei D Kolotii 1,2, Irina A Demidova 1,2, Viktor S Kravets 1,2, Oxana S Kurinnaia 1,2, Ivan Y Iourov 1,2,3
Affiliations: 1. Russian National Research Medical University named after N.I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russia;
2. Mental Health Research Center, Moscow, Russia;
3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health, Moscow, Russia

Title: Interphase quantitative FISH (IQ-FISH): implications for chromosome instability analysis
Authors: Ivan Y Iourov 1,2,3, Svetlana G Vorsanova 1,2, Ilia V Soloviev 1, Yuri B Yurov 1,2
Affiliations: 1. Mental Health Research Center, Moscow, Russia;
2. Russian National Research Medical University named after N.I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russia;
3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health, Moscow, Russia

Title: Molecular studies detected an original complex karyotype involving a KMT2A gene rearrangement in an infant with mixed lineage acute leukemia
Authors: Daniela R. Ney Garcia1,2, Roberto Rodrigues Capela de Matos1,3, Moneeb A. K. Othman4, Mariana Tavares de Souza M. Bizarro1,3, Moisés Martins da Rocha2, Gerson Moura Ferreira5, Lisandra Antonia Castro Teixeira2, Maria Luiza Macedo Silva1,2,3, Marcelo G. P. Land2, Elaine S. Costa2.
Affiliations: 1. Cytogenetics Department, Bone Marrow Transplantation Unit, National Cancer Institute (INCA), Rio de Janeiro, Brazil;
2. Clinical Medicine Post-Graduation Program, College of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;
3. Post-Graduation Program in Oncology, National Cancer Institute (INCA) Rio de Janeiro, Brazil;
4. Jena University Hospital, Institute of Human Genetics, Jena, Germany;
5. Stem Cells Department, Bone Marrow Transplantation Unit, National Cancer Institute (INCA), Rio de Janeiro, Brazil.

Title: Potential of Interphase-FISH to Evaluate Epithelial Growth Factor Receptor of MDA-MB-231 Cell line Treated with Lapatinib and Herceptin
Author: Mona A.M. Abo-Zeid 1,2,*
Affiliations: 1. Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki 12622, Cairo, Egypt.
2. Cancer Biology and Genetics Laboratory Centre of Excellence for Advanced Sciences, National Research Centre, Dokki 12622, Cairo, Egypt.

Title: sSMC characterization in a male with Turner syndrome stigmata
Authors: F. J. Sheth 1#, T. Liehr 2, J. Andrieux 3, S. Tewari 1, N. Lubna1, J. Sheth 1
Affiliations: 1. FRIGE’s Institute of Human Genetics, FRIGE House, Jodhpur Gam Road, Satellite, Ahmedabad-380 015. India;
2. Jena University Hospital, Institute of Human Genetics, Jena, Germany;
3. Laboratory of Medical Genetics, Lille, France

Title: Comparative Karyotype Analysis of Cardinalfishes (Perciformes, Apogonidae) in Thailand by Classical Cytogenetic and FISH Techniques
Authors: Wannapa Kasiroek1, Sumalee Phimphan2, Krit Pinthong3, Puan Pengseng4, Weerayuth Supiwong5 and Alongklod Tanomtong2*
Affiliations: 1. Institute of Marine Science, Burapha University, Muang, Chonburi 20131, Thailand
2. Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand
3. Department of Fundamental Science, Faculty of Science and Technology, Surindra
Rajabhat University, Muang, Surin 32000, Thailand
4. School of Agricultural of Technology, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand
5. Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Muang, Nong Khai 43000, Thailand

Title: Myelodysplastic syndrome with complex chromosomal rearrangement: about an observation and review of the literature
Authors: Hanane Merhni1,2, Wafaa Jdioui1,2, Yassamine Doubaj1,2, Abdelhafid Natiq2, Aziza Sbiti2, Thomas Lieher3, Abdelaziz Sefiani1
Affiliations: 1- Centre de Génomique Humaine, Faculté de Médecine et de Pharmacie, Rabat, Maroc
2- Département de Génétique Médicale, Institut National d’Hygiène, Rabat, Maroc
3- Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena , Germany

Title: Constitutional partial proximal trisomy 14q21: about two new Moroccan cases and review of the literature
Authors: Hanane Merhni1,2, Maria Zerkaoui1,2, Abdelhafid Natiq2, Aziza Sbiti2,Thomas Liehr3, Abdelaziz Sefiani1
Affiliations: 1- Centre de Génomique Humaine, Faculté de Médecine et de Pharmacie, Rabat, Maroc
2- Département de Génétique Médicale, Institut National d’Hygiène, Rabat, Maroc
3- Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany

Title: In silico detection and FISH location of miRNAs in Solea senegalensis chromosomes usisng BACs
Authors: Ramírez Daniel¹, Arias-Pérez Alberto¹, Rodríguez María Esther¹, Portela-Bens Silvia¹, García-Suarez Emilio¹, Merlo Manuel Alejandro¹, García-Angulo Aglaya, Cross Ismael,¹ Liehr Thomas², Rebordinos Laureana¹
Affiliations: 1. Area de Genética, Departamento de Biomedicina y Biotecnología, INMAR, UCA, Puerto Real, Cádiz, Spain.
2. Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany.