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Special Issue

Applications of Fluorescence in Situ Hybridization

Submission Deadline: July 31, 2018 (Open)                Submit Now

Guest Editor

Thomas Liehr, PhD, Dr.h.c., inv. Prof. (YSU and BMS)
University Clinic Jena, Institute of Human Genetics, Am Klinikum 1, 07747 Jena, Germany
E-Mail: [email protected]
Website: http://www.humangenetik.uniklinikum-jena.de/Forschung/Molekulare+Zytogenetik/Mitarbeiter.html
Research Interests: clinical genetics; (molecular) cytogenetics; interphase architecture; ZOO-FISH; marker and derivative chromosomes

About This Topic

Fluorescence in situ hybridization (FISH) is an approach applied and applicable in many fields of biological research and diagnostics. Its unique feature is to provide information on localization and copy numbers of specific stretches of nucleic acids on the single cell level. Thus, FISH cannot be replaced by, like in medical field at present very popular ‘high throughput approaches’, which are mostly providing information on the genetic content of thousands to millions of cells at a time. FISH provides multiple possibilities and variants, according to the question to be answered. Fluorescence in situ hybridization can be applied as (i) single to multi-color FISH approaches, (ii) using DNA, RNA, cDNA, PNA, and other types of labeled nucleotides as probes; also (iii) samples for FISH can be various human, animal or plant derived tissue types, and even single cellular organisms.

In this special issue of OBM Genetics, we have selected topics that highlight the progress, the state-of–the-art and future potential of fluorescence in situ hybridization, ranging from practical and technological aspects to applications in research and diagnostics of fluorescence in situ hybridization in human, animal, plant, fungi, biofilms and bacteria. Overall this issue provides information where and how fluorescence in situ hybridization can be applied in practice, and shows its high potential in diagnostics and research.

Planned Papers

Title: A case study of in vitro and in vivo karyotype evolution in melanoma using M-FISH
Authors: Banerjee R, Alifrangis C, Fu B, Corrie P, McDermott U and Yang F
Affiliations: Wellcome Trust Sanger Institute, Cambridge, United Kingdom; E-Mail: [email protected]

Title: Colocalization of telomeric sequences and rDNA revealed by dual-FISH
Author: Susanna Salvadori
Affiliations: Dipartimento di Scienze della Vita e dell'Ambiente, Univ Cagliari, Dept Life & Environm Sci, Via T Fiorelli 1, I-09126 Cagliari, Italy

Title: Usefulness of UroVysion test in diagnosis and monitoring of bladder cancer
Author: Edyta Borkowska
Affiliation: Medical University of Lodz, Chair of Laboratory and Clinical Genetics, Department of Clinical Genetics, Lodz, Poland

Title: A novel acquired t(2;4)(q36.1;q24) with a concurrent submicroscopic del(4)(q23q24) in an adult with polycythemia vera
Author: Eigil Kjeldsen
Affiliation: Professor and senior consultant, HemoDiagnistic Laboratory, Head of Cancer Cytogenetics Section, Aarhus University Hospital, Tage-Hansen Gade 2, Ent. 4A, DK-8000 Aarhus C, Denmark

Title: The application of FISH in melanoma diagnosis
Author: Margit Balazs
Affiliation: Department of Preventive Medicine, Faculty of Public Health, University of Debrecen, Hungary, 4028, Debrecen, Kassai str. 26/b., Hungary

Title: FISH and SCLC targeted therapy
Author: Paul Hartel
Affiliation: Davis Medical Center of Davis Health System, Elkins, West Virginia, USA

Publication

Review

FISH-Based Analysis of Mosaic Aneuploidy and Chromosome Instability for Investigating Molecular and Cellular Mechanisms of Disease

Svetlana G. Vorsanova, Yuri B. Yurov, Ilia V Soloviev, Alexei D Kolotii, Irina A Demidova, Viktor S Kravets, Oxana S Kurinnaia, Maria A Zelenova, Ivan Y. Iourov
Received: February 8, 2019; Published: March 20, 2019; doi:10.21926/obm.genet.1901068

Technical Note

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

Ivan Y. Iourov , Ilia V. Soloviev, Yuri B. Yurov, Svetlana G. Vorsanova
Received: February 2, 2019; Published: March 20,2019; doi:10.21926/obm.genet.1901067

Research Article

COMBO-FISH: A Versatile Tool Beyond Standard FISH to Study Chromatin Organization by Fluorescence Light Microscopy

Jin-Ho Lee, Florence Laure Djikimi Tchetgna, Matthias Krufczik, Eberhard Schmitt, Christoph Cremer, Felix Bestvater, Michael Hausmann
Received: September 14, 2018; Published: February 19, 2019; doi:10.21926/obm.genet.1901064

Opinion

Interphase FISH: A Helpful Assay in Prenatal Cytogenetics Diagnosis

Elena Sala, Donatella Conconi, Francesca Crosti, Nicoletta Villa, Serena Redaelli, Gaia Roversi
Received: October 1, 2018; Published: January 29, 2019; doi:10.21926/obm.genet.1901063

Technical Note

Repli-FISH (Fluorescence in Situ Hybridization): Application of 3D-(Immuno)-FISH for the Study of DNA Replication Timing of Genetic Repeat Elements

Patrick Weber, Cathia Rausch, Annina Scholl, M. Cristina Cardoso
Received: July 22, 2018; Published: January 25, 2019; doi:10.21926/obm.genet.1901062

Research Article

Assessment of Cytogenetic Abnormalities by FISH in Lymphocytes from a Victim Accidentally Exposed to Cobalt-60

Rositsa Hristova, Dimka Georgieva, Albena Staynova, Valeria Hadjidekova
Received: August 14, 2018; Published: November 26, 2018; doi:10.21926/obm.genet.1804051

Case Report

Increased Recurrence Risk in Phelan-McDermid (22q13.3 Deletion) Syndrome: the Importance of FISH Demonstrated by a Case Series of Five Families

Renée J. Zwanenburg, Trijnie Dijkhuizen, Martijn J. de Groot, Sheela Nampoothiri, Marjolein H. Willemsen, Eelco Dulfer, Madhavan V. Thampi, Nicole de Leeuw, Conny M. A. van Ravenswaaij-Arts
Received: July 13, 2018; Published: November 22, 2018; doi:10.21926/obm.genet.1804050

Original Research

In Silico Detection and FISH Analysis to Determine Location of miRNAs in Solea senegalensis Chromosomes Using BACs

Alberto Arias-Pérez, Daniel Ramírez-Torres, María E. Rodríguez, Silvia Portela-Bens, Emilio García-Suarez, Manuel A. Merlo, Aglaya García-Angulo, Ismael Cross, Thomas Liehr, Laureana Rebordinos
Received: July 31, 2018; Published: October 24, 2018; doi:10.21926/obm.genet.1804044

Research Article

Micronucleus-Centromere Assay to Measure Ionizing Radiation Damage of Low Dose Occupational Exposure

Albena Staynova, Ljubomira Hadjiiska, Valeria Hadjidekova, Rositsa Hristova
Received: August 14, 2018; Published: October 22, 2018; doi:10.21926/obm.genet.1804043

Book Review

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Adayabalam S. Balajee
Received: August 26, 2018; Published: October 19, 2018; doi:10.21926/obm.genet.1804042

Technical Note

Quality Assurance/Quality Control of Fluorescence in Situ Hybridization Tests in Hematologic Malignancies

Zhenya Tang, Jun Gu, Guilin Tang, L. Jeffrey Medeiros
Received: July 22, 2018; Published: October 3, 2018; doi:10.21926/obm.genet.1804038

Original Research

Molecular Cytogenetic Characterization of Two Murine Colorectal Cancer Cell Lines

Hans Rhode, Thomas Liehr, Nadezda Kosyakova, Martina Rinčić, Shaymaa S. Hussein Azawi
Received: July 30, 2018; Published: September 20, 2018; doi:10.21926/obm.genet.1803037

Case Report

Identification of a Small Supernumerary Marker Chromosome Involving 11p14.1q12.1 in a Prenatal Case: Clinical and Molecular Characterization

Maria Kontodiou, Vassilis Paspaliaris, Themistoklis Dagklis, Elisavet Siomou, Ahmed Hamid Al-Rikabi, Kalliopi Tsita, Theano Stavroulaki, Andreas Pampanos, Apostolos Zavlanos, Georgios Papaioannou, Ioannis Papoulidis, Loretta Thomaidis, Emmanouil Manolakos
Received: May, 30, 2018; Published: September 18, 2018; doi:10.21926/obm.genet.1803035

Case Report

sSMC Characterization in a Male with Turner Syndrome Stigmata

Frenny Sheth, Thomas Liehr, Kristin Mrasek, Joris Andrieux, Stuti Tewari, Naznin Lubna, Jayesh Sheth
Received: March 23, 2018; Published: September 07, 2018; doi:10.21926/obm.genet.1803033

Technical Note

User-Friendly Approach to Gain Isolation of Interphase Cells for Fluorescence in Situ Hybridization

Thomas Liehr, Monika Ziegler, Nadezda Kosyakova, Ahmed B.H. Al-Rikabi
Received: March 16, 2018; Published: May 18, 2018; doi:10.21926/obm.genet.1802021