Successive Detection of Telomerase by IHC and Assessment of Telomere Length by Q-FISH in Paucicellular Cumulus Samples from Cumulus-Oocyte Complexes Obtained in Assisted Reproduction Programs
Abstract
(ISSN 2577-5790)
OBM Genetics is an international Open Access journal published quarterly online by LIDSEN Publishing Inc. It accepts papers addressing basic and medical aspects of genetics and epigenetics and also ethical, legal and social issues. Coverage includes clinical, developmental, diagnostic, evolutionary, genomic, mitochondrial, molecular, oncological, population and reproductive aspects. It publishes a variety of article types (Original Research, Review, Communication, Opinion, Comment, Conference Report, Technical Note, Book Review, etc.). There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.
Publication Speed (median values for papers published in 2023): Submission to First Decision: 5.1 weeks; Submission to Acceptance: 17.0 weeks; Acceptance to Publication: 7 days (1-2 days of FREE language polishing included)
Special Issue
Applications of Fluorescence in Situ Hybridization II.
Submission Deadline: September 30, 2024 (Open) Submit Now
Guest Editor
Thomas Liehr, PhD, Dr.h.c., ass. Prof. (YSU and BMS)
University Clinic Jena, Institute of Human Genetics, Am Klinikum 1, 07747 Jena, Germany
Research Interests: clinical genetics; (molecular) cytogenetics; interphase architecture; ZOO-FISH; marker and derivative chromosomes
About This Topic
Fluorescence in situ hybridization (FISH) is an approach applied and applicable in many fields of biological research and diagnostics. Its unique feature is to provide information on localization and copy numbers of specific stretches of nucleic acids on the single cell level. Thus, FISH cannot be replaced by, like in medical field at present very popular ‘high throughput approaches’, which are mostly providing information on the genetic content of thousands to millions of cells at a time. FISH provides multiple possibilities and variants, according to the question to be answered. Fluorescence in situ hybridization can be applied as (i) single to multi-color FISH approaches, (ii) using DNA, RNA, cDNA, PNA, and other types of labeled nucleotides as probes; also (iii) samples for FISH can be various human, animal or plant derived tissue types, and even single cellular organisms.
In this special issue of OBM Genetics, we have selected topics that highlight the progress, the state-of–the-art and future potential of fluorescence in situ hybridization, ranging from practical and technological aspects to applications in research and diagnostics of fluorescence in situ hybridization in human, animal, plant, fungi, biofilms and bacteria. Overall this issue provides information where and how fluorescence in situ hybridization can be applied in practice, and shows its high potential in diagnostics and research.
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Publication
Successive Detection of Telomerase by IHC and Assessment of Telomere Length by Q-FISH in Paucicellular Cumulus Samples from Cumulus-Oocyte Complexes Obtained in Assisted Reproduction Programsby
Anna Pendina
,
Mikhail Krapivin
,
Irina Mekina
,
Irina Aleksandrova
,
Yanina Sagurova
,
Evgeniia Komarova
,
Mariia Ishchuk
,
Andrei Tikhonov
,
Olesya Bespalova
,
Alexander Gzgzyan
,
Igor Kogan
and
Olga Efimova
Abstract This paper suggests an approach for the use of a single paucicellular histological sample to investigate two characteristics indicative of the cell’s functional potential: 1) the content of telomerase reverse transcriptase (TERT) and 2) the telomere length. An algorithm has been suggested for the successive detection of the catal [...] |
First Cytogenomic Characterization of the Murine Testicular Tumor Cell Line I-10Abstract After being established in 1967, the commercially available murine Leydig cell tumor line I-10 has been used in almost 50 published studies. I-10 has not been characterized, either at the chromosomal/ cytogenetic level or the genetic level, similar to many other murine tumor cell lines. In this study, we performed molecular karyotyping and [...] |
Application of the FISH Method for Analyzing Chromosome Segregation Patterns in Preimplantation Embryos from Robertsonian Translocation Carriersby
Irina Puppo
,
Ziravard Tonyan
,
Alisa Panina
,
Ksenia Shunkina
,
Alsu Saifitdinova
,
Yulia Loginova
,
Anna Kinunen
,
Julia Pastuhova
,
Olga Leonteva
,
Olga Chiryaeva
,
Olga Маlysheva
,
Elena Fedorova
,
Tatyana Vavilova
and
Natalia Bichevaya
Abstract Robertsonian translocations (RTs) are very common balanced structural chromosome rearrangements in humans. Due to alterations in the chromosome segregation pattern and the formation of unbalanced gametes and embryos, the carriers of RTs have a significant risk of reproductive failure. For over 30 years, fluorescent in situ hybridization (FISH [...] |
Electroporation-Based Non-Viral Gene Delivery to Adipose Tissue in Miceby
Masahiro Sato
,
Issei Saitoh
,
Yuki kiyokawa
,
Eri Akasaka
,
Shingo Nakamura
,
Satoshi Watanabe
and
Emi Inada
Abstract Adipose tissue is distributed throughout the body as fat depots. The amount of adipose tissue increases with age. In mice, epididymal fat depots in males and gonadal fat depots in females are associated with the reproductive system. Regarding fat depots [...] |
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