TY  - JOUR
AU  - Amjad, Elham
AU  - Sokouti, Babak
PY  - 2025
DA  - 2025/06/09
TI  - Integrating RNA-Seq Biomarkers and Diagnostic Algorithms to Improve Liver Transplant Outcomes: A Comprehensive Review
JO  - OBM Transplantation
SP  - 251
VL  - 09
IS  - 02
AB  - Graft rejection, early allograft dysfunction (EAD), and poor diagnostic accuracy are some of the challenges that still need to be addressed, even though liver transplantation (LT) has the potential to help patients with end-stage liver disease (ESLD) significantly. Traditional procedures, such as liver biopsies and liver function tests (LFTs), often fail to identify abnormalities early and with accuracy. Genomic RNA sequencing (RNA-Seq) has recently emerged as a powerful approach for identifying molecular markers of immune activity and graft healing. This study provides a comprehensive review of the current achievements in RNA-Seq applications for liver transplantation by comparing biomarker profiles of liver biopsies and peripheral blood mononuclear cells (PBMCs). We highlight the success and challenges of integrating RNA-seq into clinical processes by critically examining its consistency, diagnostic importance, and translational potential. Furthermore, we evaluated the possibility of novel diagnostic algorithms and multi-omics techniques for improving early diagnosis, risk profiling, and personalized immunosuppression. This study highlights the gaps in cross-cohort repeatability, clinical validation, and standardization to improve long-term transplant outcomes. This highlights the need for integrated multicenter approaches driven by biomarkers and provides recommendations for further studies.
SN  - 2577-5820
UR  - https://doi.org/10.21926/obm.transplant.2502251
DO  - 10.21926/obm.transplant.2502251
ID  - Amjad2025
ER  -