TY  - JOUR
AU  - Ye, Qing
AU  - Wu, Yijen
AU  - Moley, Lesley
AU  - Mills, Parker
AU  - Yeh, Fang-Cheng
AU  - Zhang, Haosen
AU  - Eytan, Danielle
AU  - Liu, Li
AU  - Hitchens, T
AU  - Ho, Chien
PY  - 2019
DA  - 2019/08/29
TI  - Characterization of Early Indicators of Cardiac Allograft Vasculopathy Lesions in a Rat Model Using Non-Invasive Cellular MR
JO  - OBM Transplantation
SP  - 083
VL  - 03
IS  - 03
AB  - Background:  Cardiac allograft vasculopathy (CAV) remains a major obstacle to long-term heart allograft survival. A number of studies show that immune mechanisms involved in CAV.  Using non-invasive cellular MRI (CMRI) to explore indicators of CAV lesions and characterize its development could provide new insights into the target immune cells that are responsible for the progression of CAV and reveal early markers of the disease before irreversible changes occur. 
Methods and Results: Rat heart transplant allografts (n = 88) and isografts (n = 22) were employed in this study.  CMRI at 4.7-Tesla revealed a few hypointense foci in the graft early on during CAV with more regions becoming involved as the disease progressed. The areas with hypointensity corresponded with infiltrating ED1+ cells labeled with micrometer-sized superparamagnetic iron oxide (MPIO), confirmed by MR microscopy (MRM) at 11.7-Tesla and pathology.  MRI abnormalities of the transplanted hearts correlate well with the histopathological findings.  MPIO-labeled cells counted by a computer algorithm that analyzed 3D MRM volumes exhibited a strong correlation with those counted by immunohistochemical inspection of ED1+ cell infiltration (R2 = 0.8075).  Histology found that in the early phases of CAV, lesions were focal and mostly began in the adventitia.  Immunohistochemistry indicated that the infiltrates were mostly ED1+ cells and that their density was significantly correlated with the severity of CAV (p < 0.05).
Conclusions: This study illustrates the feasibility of monitoring MPIO-labeled ED1+ cells as an early indicator for CAV before vessel wall changes using non-invasive CMRI, which provides information on the entire heart.
SN  - 2577-5820
UR  - https://doi.org/10.21926/obm.transplant.1903083
DO  - 10.21926/obm.transplant.1903083
ID  - Ye2019
ER  -