TY  - JOUR
AU  - Christmas, Stephen E
AU  - Hammad, Abdul
AU  - Flanagan, Brian
AU  - Middleton, Derek
AU  - Albayati, Zaid
AU  - Heyworth, Sally
AU  - Moberly, Jane
PY  - 2018
DA  - 2018/12/14
TI  - Changes in HLA-G Expression by Leucocyte Subsets Following Renal Transplantation and the Effect of Immunosuppressive Drugs on Leucocyte HLA-G Expression in Vitro
JO  - OBM Transplantation
SP  - 032
VL  - 02
IS  - 04
AB  - Background:HLA-G is an immunosuppressive molecule with a potential role in allograft acceptance.
Methods:Expression of HLA-G on leucocyte subpopulations was studied in a group of 21 renal transplant patients immediately prior to and 2 months post-transplantation.
Results:Significant increases in proportions of total HLA-G+CD4+ T cells were seen (0.5+/-0.1% to 7.9+/-3.2%; p<0.01) and also for CD45RA+, CD45RO+ and CD69+ subsets. Increases in proportions of HLA-G+ cells were also seen in CD8+ T cells and their subsets, CD56+ T cells, B cells, NK cells and monocytes, and also of serum soluble HLA-G, but these did not reach statistical significance. When leucocytes from healthy subjects were cultured for 7 days in therapeutic concentrations of tacrolimus and mycophenolate mofetil, no significant increases in proportions of HLA-G+CD4 or CD8+ T cells were found while those for CD56+ T cells (3.4+/-0.2% to 13.0+/-0.4%) and B cells (1.6+/-0.1% to 6.4+/-0.2%) increased significantly (p<0.01). Proportions of HLA-G+CD103+ B cells, NK cells and CD56+ T cells, but not classical T cells, were also significantly increased (p<0.05) following culture in immunosuppressive drugs.
Conclusions: The results show that HLA-G is upregulated in leucocyte subsets following renal transplantation but that in CD4+ and CD8+ T cells this is not directly mediated by immunosuppressive drugs.
SN  - 2577-5820
UR  - https://doi.org/10.21926/obm.transplant.1804032
DO  - 10.21926/obm.transplant.1804032
ID  - Christmas2018
ER  -