TY  - JOUR
AU  - Stephens, Dylan J.
AU  - Johnson, Evan B.
AU  - Johnson, Bradley J.
PY  - 2026
DA  - 2026/07/30
TI  - Dose-Dependent Effects of Berberine Chloride Dihydrate on Proliferation of C2C12 Myoblasts and Differentiation of 3T3L1 Adipocytes
JO  - Recent Progress in Nutrition
SP  - 018
VL  - 06
IS  - 03
AB  - Berberine is an alkaloid chemical found in plants, including Berberidaceae, Coptis rhizomes, and Hydrastis canadensis. Berberine has been shown to improve insulin sensitivity, promote adipocyte loss, and is associated with higher levels of skeletal muscle protein synthesis. The purpose of this study was to examine berberine’s dose-dependent effects on C2C12 myoblast proliferation and 3T3L1 adipocyte differentiation. Cells were grown out over 3 weeks, and once confluent at 90%, they were treated with berberine to examine its effect on cell survival and growth. One-way ANOVA was used to analyze differences between groups. Results showed that a 10 µM dose of berberine had an optimal effect on C2C12 myoblast proliferation at 72 hours (p < 0.01). In contrast, after 48 hours of inoculation, 10 µM and 100 µM doses of berberine showed suppressive effects on 3T3L1 adipocyte cell counts (p = 0.00035). In conclusion, berberine appeared to be advantageous for C2C12 myoblasts’ survival, whereas 3T3L1 adipocytes suffered in its presence after 48 hours of inoculation.
SN  - 2771-9871
UR  - https://doi.org/10.21926/rpn.2603018
DO  - 10.21926/rpn.2603018
ID  - Stephens2026
ER  -