TY - JOUR AU - Ravindranath, Rohan R. AU - Yougbare, Issaka AU - Ni, Heyu AU - Fish, Jason E. AU - Romaschin, Alexander AU - Thompson, Michael PY - 2020 DA - 2020/03/27 TI - Antibody-Based Capture and Behaviour of Endothelial Cell Lines on Pre-Surface Modified Medical Grade Steel JO - Recent Progress in Materials SP - 008 VL - 02 IS - 01 AB - Coronary artery disease is one of the major causes of morbidity and mortality worldwide. Coronary stents, tube-shaped medical implants that are placed in narrowed coronary arteries, have been used successfully in the management of this condition. However, re-narrowing (i.e. restenosis) of the artery can occur which is instigated by an immune response towards the implanted ‘foreign’ material. A new approach to prevent restenosis and reduce the stent-induced immune response has been proposed previously, which involves re-endothelialization of the implanted stent. In the present study a proof-of-concept experiment involving surface-modified medical grade steel was employed in order to examine the best surface chemistry for in vitro cell capture. Steel coupons were coated with a silanized adlayer, followed by attachment of whole antibodies, followed by culturing of human umbilical vein endothelial cells (HUVECs) or human aortic endothelial cells (HAECs or HAoECs). With regard to the adlayer-antibody configuration, HUVECs adhered and grew with normal morphology, and a significantly increased number of HUVECs proliferated on the coupons compared to the ‘bare’ surface. Similar effects were observed with HAECS grown on adlayer-antibody modified substrates, with a significantly higher number of cells proliferating. These results demonstrate a successful strategy for re-endothelialization of the steel surface that may prevent immune response with respect to the behaviour of steel-based stents in vivo. SN - 2689-5846 UR - https://doi.org/10.21926/rpm.2001008 DO - 10.21926/rpm.2001008 ID - Ravindranath2020 ER -