TY  - JOUR
AU  - Blazon, Maxwell
AU  - LaCarubba, Brianna
AU  - Bunda, Alexandra
AU  - Czepiel, Natalie
AU  - Londrigan, Laura
AU  - Mallat, Shayna
AU  - Andrade, Arturo
PY  - 2021
DA  - 2021/01/12
TI  - N-Type Calcium Channels Control GABAergic Transmission in Brain Areas related to Fear and Anxiety
JO  - OBM Neurobiology
SP  - 083
VL  - 05
IS  - 01
AB  - N-type (CaV2.2) calcium channels are key for action potential-evoked transmitter release in the peripheral and central nervous system. Previous studies have highlighted the functional relevance of N-type calcium channels at both the peripheral and central level. In the periphery, N-type calcium channels regulate nociceptive and sympathetic responses. At the central level, N-type calcium channels have been linked to aggression, hyperlocomotion, and anxiety. Among the areas of the brain that are involved in anxiety are the basolateral amygdala, medial prefrontal cortex, and ventral hippocampus. These three areas share similar characteristics in their neuronal circuitry, where pyramidal projection neurons are under the inhibitory control of a wide array of interneurons including those that express the peptide cholecystokinin. This type of interneuron is well-known to rely on N-type calcium channels to release GABA in the hippocampus, however, whether these channels control GABA release from cholecystokinin-expressing interneurons in the basolateral amygdala and medial prefrontal cortex is not known. Here, using mouse models to genetically label cholecystokinin-expressing interneurons and electrophysiology, we found that in the basolateral amygdala, N-type calcium channels control ~50% of GABA release from these neurons onto pyramidal cells. By contrast, in the medial prefrontal cortex N-type calcium channels are functionally absent in synapses of cholecystokinin-expressing interneurons, but control ~40% of GABA release from other types of interneurons. Our findings provide insights into the precise localization of N-type calcium channels in interneurons of brain areas related to anxiety.
SN  - 2573-4407
UR  - https://doi.org/10.21926/obm.neurobiol.2101083
DO  - 10.21926/obm.neurobiol.2101083
ID  - Blazon2021
ER  -