TY  - JOUR
AU  - Burda, Rastislav
AU  - Danielisová, Viera
AU  - Burda, Jozef
PY  - 2019
DA  - 2019/09/20
TI  - The End Effector of Ischemic Tolerance Present in Blood Plasma from Double Conditioned Donors Ameliorates Trimethyltin Provoked Damage in Brain
JO  - OBM Neurobiology
SP  - 041
VL  - 03
IS  - 03
AB  - Background: Many experiments have been done to demonstrate robust ischemic tolerance efficiency using mostly young and healthy animals. However, the translation of these results to usually elderly and sick patients moreover taking many various medicines has to date been disappointing. 3-Methyltin (TMT) poisoning and short-term transient cerebral ischemia cause similar damage, especially, to selectively vulnerable brain regions such as hippocampal CA1 and CA3.
Methods: Using dual conditioning, we activated the full ischemic tolerance the products of which are located to blood plasma. As sublethal stresses, two periods of 20-minute hind-limb ischemia was used with a two-day interval in rats. Active plasma was isolated 6 h after the second hind limb ischemia. Brain damage was detected by visualizing dead neurons by Fluoro-Jade B staining and neuronal survival by NeuN immunoreaction. The functional capabilities of the surviving neurons were monitored by the Morris water maze.
Results: The use of activated plasma dramatically reduced the hippocampal degenerative CA1 neurons from 56.59% to 7.51%. NeuN positivity was increased from 23.82% to 78.67%. Time spent searching for a hidden island in the Morris water maze was shortened from 48.46 to 21.63 s.
Conclusions: By using double conditioning it is possible to provoke the synthesis of substances present in the blood plasma, providing the organism with significant protection against acute degenerative processes.
SN  - 2573-4407
UR  - https://doi.org/10.21926/obm.neurobiol.1903041
DO  - 10.21926/obm.neurobiol.1903041
ID  - Burda2019
ER  -