TY  - JOUR
AU  - Dara, Mahintaj
AU  - Azarpira, Negar
AU  - Motazedian, Nasrin
AU  - Hossein-Aghdaie, Mahdokht
AU  - Dehghani, Seyed-Mohsen
AU  - Geramizadeh, Bita
AU  - Esfandiari, Elaheh
PY  - 2025
DA  - 2025/11/26
TI  - Differential Expression of MiR-21 and MiR-19 in Biliary Atresia: Diagnostic Potential and Therapeutic Implications
JO  - OBM Genetics
SP  - 319
VL  - 09
IS  - 04
AB  - Biliary atresia (BA), a severe pediatric liver disease of unknown etiology, causes neonatal jaundice, progressive cholestasis, and life-threatening liver fibrosis. Emerging evidence suggests microRNAs (miRNAs) – small non-coding RNAs that regulate gene expression by binding target mRNAs may play a role in BA pathogenesis. Circulating miRNAs have shown diagnostic potential for various diseases, prompting our investigation of miR-21 and miR-19 as potential biomarkers in BA. We conducted a case-control study comparing 18 BA patients from the Shiraz Pediatric Liver Cirrhosis Cohort Study (SPLCCS) with 18 age-matched healthy controls. Serum samples were subjected to RNA extraction, followed by cDNA synthesis with stem-loop primers. Quantitative real-time PCR was performed to measure miR-21 and miR-19 expression levels, with U6 snRNA as an endogenous control. Quantitative PCR analysis revealed distinct miRNA expression patterns in BA patients compared to healthy controls. miR-21 expression was significantly elevated in the BA group, demonstrating a 5-fold increase relative to controls (p = 0.0002). Conversely, miR-19 showed a 2.1-fold downregulation (0.47-fold change) in BA patients, though this decrease did not reach statistical significance (p = 0.2118). Quantitative PCR analysis revealed distinct miRNA expression patterns in BA patients compared to healthy controls. miR-21 expression was significantly elevated in the BA group, demonstrating a 5-fold increase relative to controls (p = 0.0002). Conversely, miR-19 showed a 2.1-fold downregulation (0.47-fold change) in BA patients, though this decrease did not reach statistical significance (p = 0.2118).
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.2504319
DO  - 10.21926/obm.genet.2504319
ID  - Dara2025
ER  -