TY  - JOUR
AU  - Starodub, Lyubov
AU  - Mokhnachova, Nataliia
AU  - Zhukorskyi, Ostap
PY  - 2025
DA  - 2025/07/07
TI  - Evaluation of Polylocus Spectra of <i>DNA</i> Fragments of the <i>Genus Eguus</i> Using <i>ISSR-PCR</i> Markers
JO  - OBM Genetics
SP  - 302
VL  - 09
IS  - 03
AB  - Characterization of the non-coding part of the transcriptome is a supplement to the annotation of the genome of living organisms. According to ISSR-fingerprinting data, information is obtained about ancient gene pools, modern gene pools, genetic variability and conservation of breeds and species of farm animals. The aim of the study is to study the molecular genetic component and phylogenetic relationships in the aboriginal Hutsul horse breed and fossil remains of ancient horses using ISSR-PCR markers. The polymorphism of amplified DNA fragments isolated from the blood of Hutsul horses and fossil bones of ancient equids, flanked by nucleotide repeats ((ACC)6G, (CTC)6C, (GAG)6C, (GA)6CC, (AG)8CG, (AG)8CA, (GA)9C and (AG)9C), was investigated using the ISSR-PCR labeling method. Genotyping of 30 Hutsul horses revealed 54 loci, 33 of which are polymorphic. The average value of the index, which shows the level of informativeness of the locus, was 0.31. Fragments with a size of 380-400, 500-520 and 600-630 nucleotide pairs are species-specific for the genus Eguus, amplicons with a size of 340-350 nucleotide pairs of primer (GA)9C and 470-490 primers (GA)9C and (GA)6CC were found only in fossil remains. The closest genetic distances were between the true tarpan and the ancient domestic horse - 0.0234; between the Pleistocene horse and the ancient domestic horse - 0.0385; the most distant were between the Hutsul horses and the true tarpan - 0.5057. Using ISSR-markers, a moderate level of genetic diversity of Hutsul horses was established. As a result of the research, unique spectra were found for the genus Equus and for ancient equids. Genetic distances were calculated to reflect the genetic relationships between the studied horses and fossil remains. The closest were the tarpan and the ancient domestic horse.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.2503302
DO  - 10.21926/obm.genet.2503302
ID  - Starodub2025
ER  -