TY  - JOUR
AU  - Zirak, Leila
AU  - Khakvar, Reza
PY  - 2023
DA  - 2023/12/04
TI  - Characterization of ‘<em>Ca</em>. Phytoplasma asteris’, ‘<em>Ca</em>. Phytoplasma australiense’ and ‘<em>Ca</em>. Phytoplasma mali’ Associated with Russian Olive Witches’-Broom Disease in Iran Using Next-Generation Sequencing
JO  - OBM Genetics
SP  - 204
VL  - 07
IS  - 04
AB  - Russian olive trees showing witches’-broom disease symptoms in urban green spaces and orchards in northwest Iran were sampled for phytoplasma detection. PCR assays and Sanger sequencing of 16S rRNA gene confirmed that ‘Ca. Phytoplasma asteris’ was associated with Russian olive witches’-broom disease. For genomic characterization of phytoplasma associated with disease, the total DNA of an infected tree was analyzed by Illumina next-generation sequencing (NGS). The NGS analysis generated 46011389 read pairs of raw data with about 6.8 × 109 bp sequences and 31% GC content. Then, MetaPhlAn2 analysis of NGS raw data predicted three phytoplasma species inside the infected Russian olive tree DNA. Finally, the genomes of ‘Ca. Phytoplasma asteris’ with 833199 bp length, ‘Ca. Phytoplasma australiense’ with 762261 bp length and ‘Ca. Phytoplasma mali’ with 518942 bp length were obtained from NGS raw data. This study represents the first report of ‘Ca. Phytoplasma australiense’ and ‘Ca. Phytoplasma mali’ association with Russian olive witches’-broom disease using NGS analyses worldwide.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.2304204
DO  - 10.21926/obm.genet.2304204
ID  - Zirak2023
ER  -