TY  - JOUR
AU  - Yurov, Yuri B.
AU  - Vorsanova, Svetlana G.
AU  - Iourov, Ivan Y.
PY  - 2019
DA  - 2019/04/30
TI  - FISHing for Unstable Cellular Genomes in the Human Brain
JO  - OBM Genetics
SP  - 076
VL  - 03
IS  - 02
AB  - The human brain has been repeatedly shown to exhibit intercellular/somatic genomic variations at the chromosomal level, which are involved in the neuronal diversity in health and disease. Brain-specific chromosomal mosaicism (aneuploidy) and chromosome instability play a role in the normal and pathological neurodevelopment, neurodegeneration and aging of the central nervous system. Regardless of achievements in somatic cell (single-cell) genomics, there is still no consensus on the amounts of chromosomally abnormal cells in the normal and diseased brain. Actually, the results of single-cell whole genome analysis seem to be different from molecular neurocytogenetic data obtained by fluorescence in situ hybridization (FISH). In this context, a review of FISH-based approaches to chromosomal mosaicism/instability in single neural cells appears to be important in the so-called post-genomic era. Looking through the literature highlighting the patterns of chromosomal mosaicism/instability in the diseased human brain, we have found that FISH-based techniques for studying interphase chromosomes represent a unique methodology for uncovering structural and behavioral genome changes in single neural cells. More importantly, interphase FISH techniques applied for molecular neurocytogenetic analysis are not interchangeable (i.e. each one is developed to solve a specific task). Therefore, it is highly likely that molecular neurocytogenetic studies will benefit from the application of FISH, leading to discoveries of neurogenomic mechanisms of human neuronal diversity and brain diseases.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.1902076
DO  - 10.21926/obm.genet.1902076
ID  - Yurov2019
ER  -