TY  - JOUR
AU  - Mochizuki, Kazuki
AU  - Osaki, Ayumi
AU  - Inamochi, Yuko
AU  - Goda, Toshinao
PY  - 2018
DA  - 2018/10/30
TI  - The Induction of Histone H3K4 Methylation on the <em>SI</em> Gene Correlates with <em>SI</em> mRNA Levels in Enterocyte-Like Caco-2 Cells
JO  - OBM Genetics
SP  - 046
VL  - 02
IS  - 04
AB  - Background: Histone modifications, including acetylation and H3 lysine 4 (K4) methylation, are thought to be associated with transcriptional activation during differentiation. Methods: mRNA and histone modifications around the sucrase-isomaltase (SI) gene in Caco-2 cells (a small intestine cell line) during differentiation were determined by qRT-PCR and chromatin immunoprecipitation, respectively. Results: Mono-, di-, and tri-methylation of histone H3K4 on the SI gene correlated with the induction of SI gene expression, RNA polymerase II binding, and acetylation of histones H3 and H4 close to the transcription initiation site during differentiation in Caco-2 cells. Monomethylation was prominent in the promoter–enhancer region, while dimethylation was observed from the promoter–enhancer region throughout the gene body region. Trimethylation was detected from the transcription initiation site throughout the gene body region. Conclusions: Histone H3K4 methylation was associated with SI gene induction during differentiation in Caco-2 cells. Moreover, the mono-, di-, and tri-methylation of histone H3K4 at different regions of the SI gene may play different roles in SI gene induction.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.1804046
DO  - 10.21926/obm.genet.1804046
ID  - Mochizuki2018
ER  -