TY  - JOUR
AU  - Balajee, Adayabalam S.
PY  - 2018
DA  - 2018/10/19
TI  - Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring
JO  - OBM Genetics
SP  - 042
VL  - 02
IS  - 04
AB  - The technique of in situ hybridization (ISH) using radioactively labeled DNA probes was first described in the late 1960s and early 1970s. The first use of fluorescence in situ hybridization (FISH) was reported in 1980s where RNA labeled with a fluorophore at the 3’ end was used to detect specific DNA sequences. Since then, the technique has undergone various modifications for detecting single genes, chromosomes and whole genome on various targets such as interphase nucleus, prematurely condensed chromosomes, metaphase chromosomes, fresh and paraffinised tissue sections. Although FISH is most widely used in clinical diagnostics, its use in the field of radiation biodosimetry has been steadily increasing where both stable (translocations and inversions) and unstable aberrations (dicentrics and rings) are detected with high resolution for estimating the absorbed radiation dose in humans after incidental, accidental or occupational exposure to ionizing radiation. This review summarizes the diverse applications of FISH in radiation biodosimetry that range from radiation dose estimation to prediction of stochastic effects of radiation such as cancer in human population.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.1804042
DO  - 10.21926/obm.genet.1804042
ID  - Balajee2018
ER  -