TY  - JOUR
AU  - Liehr, Thomas
AU  - Al-Rikabi, Ahmed B.H.
AU  - Kosyakova, Nadezda
AU  - Ziegler, Monika
PY  - 2018
DA  - 2018/05/17
TI  - User-Friendly Approach to Gain Isolation of Interphase Cells for Fluorescence in Situ Hybridization
JO  - OBM Genetics
SP  - 021
VL  - 02
IS  - 02
AB  - (1) Background: Fluorescence in situ hybridization (FISH) may be performed on metaphase- and/or interphase cells. Metaphase cells can exclusively be obtained and studied after time consuming cell culture. Therefore suited cells derive from peripheral blood, bone marrow or fibroblasts, the latter including skin, tumour or “prenatal tissues”, like amnion or chorion. On the other hand interphase cells can be gained from many different cells and even without cell culture. 
(2) Methods: Here we report an approach by which interphase cells can be gained easily from skin surface, practically without causing any pain or damage to the patient. The procedure is simple and start by adding some microliter of liquid to the patient’s skin (e.g. the forearm); subsequently the edge of a slide is used to collect skin cells together with the fluid by carefully scratching along the wetted skin region. The collected fluid is distributed on the slide surface and air dried. 
(3) Results: Applying this simple cell collection approach, several hundreds of skin-cells are obtained. The result may be checked in phase contrast microscope and regions with highest density of cells may be selected for interphase-FISH. Here we provide prove of principle of this test. Also the cells obtained in the described way can be used for DNA-based molecular genetic tests. 
(4) Conclusions: A new and straightforward method for acquisition of interphase cells is presented. It may be used to complement the available possibilities for the analyses of different tissues in case of questionable low level mosaics detected in peripheral blood testing.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.1802021
DO  - 10.21926/obm.genet.1802021
ID  - Liehr2018
ER  -