TY  - JOUR
AU  - Ribeiro, Geise Camila
AU  - Fernandes, Pedro
AU  - Costa, Floriatan Santos
AU  - de Assis, Sandra Aparecida
PY  - 2026
DA  - 2026/08/21
TI  - Stabilization Strategies of β-glucosidases Produced by Yeasts <i>Rhodotorula oryzicola</i> and <i>Saccharomyces cerevisiae</i>
JO  - Catalysis Research
SP  - 010
VL  - 06
IS  - 03
AB  - The rising demand for enzymes with improved properties has driven continuous developments in enzyme technology, including enzymatic formulation. Enzyme formulation primarily aims to minimize losses in enzymatic activity during transport, storage and use. One strategy to mitigate enzyme inactivation is to include stabilizing agents or metal ions that preserve enzyme structure and activity. Thus, in this work, we evaluated stabilization strategies for β-glucosidase produced by the yeasts Rhodotorula oryzicola and Saccharomyces cerevisiae using various chemical additives. The most effective additives were then applied to obtain a formulation with improved enzymatic activity. KCl, ZnSO4, EDTA, SDS, Triton X-100, Tween 20 and Tween 80 were tested as potential stabilizers for β-glucosidase. The three best stabilizers for each enzymatic sample were used in a simplex-centroid mixture design to evaluate possible synergistic effects and obtain a stable enzyme formulation. The formulations that yielded the highest enzymatic activity contained 2.5 mM of ZnSO4 and 0.375% of Tween 20 for β-glucosidase from R. oryzicola, and 0.5 mM of ZnSO4 and 2.5 mM of EDTA for the enzyme from S. cerevisiae, showing relative activities of 313.60% and 302.11%, respectively. The results obtained in our study show that both yeasts produce β-glucosidases with good tolerance to chemical additives, and that some of these additives can be used to obtain more stable enzymatic formulations with enhanced catalytic efficiency.
SN  - 2771-490X
UR  - https://doi.org/10.21926/cr.2603010
DO  - 10.21926/cr.2603010
ID  - Ribeiro2026
ER  -