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Journal Flyer

Current issue: 2018

Editor-in-Chief of OBM Hepatology and Gastroenterology

Osamu Yokosuka is an Emeritus Professor of Chiba University, Japan. He graduated from Chiba University School of Medicine in 1975 then worked as a trainee under Professor K. Okuda in Chiba University Hospital till 1978. Dr. Yokosuka was a research fellow worked under Professor S. Scherlock and Professor B. H. Billing in Royal Free Hospital, London, UK from 1978 to 1980; under Professor M. Omata in Chiba University from 1980 to 1985; and under Dr. J Summers in Fox Chase Cancer Center, PA, USA in 1984. In 1985, he received a Degree of Doctor of Medical Science, and served as an Assistant Professor in Chiba University till 1994, then as Lecturer in Medicine till 2006 when he was appointed as Director and Professor of Medicine. From 2013 to 2015, he served as the Dean of Chiba University School of Medicine.

Dr. Yokosuka was the Secretary General of APASL (2008-2014). In 2016, he was elected as the President of APASL Tokyo, the President of 52nd Annual Meeting of Japan Society of Hepatology, and the President of Funabashi Central Hospital. Dr. Yokosuka’s research mainly focuses on Hepatitis and Hepatocellular Carcinoma. So far, he has published more than 700 original papers.


The Associate Editor of OBM Hepatology and Gastroenterology

Tatsuo Kanda received a medical degree in 1991 at Niigata University School of Medicine, Japan, and his PhD in 1999 at Chiba University Graduate School of Medicine, Japan. He had post-doctor training for 3 years under Prof. Ratna Ray and Prof. Ranjit Ray at Saint Louis University, St. Louis, MO, USA. In Dec. 2008, Tatsuo Kanda became a Tenure-track Associate Professor at Department of Medicine and Clinical Oncology, Chiba University, Graduate School of Medicine, Japan. In Feb. 2013, Tatsuo Kanda was nominated a permanent Associate Professor at Department of Gastroenterology and Nephrology, Chiba University, Graduate School of Medicine, Japan. In 2017, Tatsuo Kanda became an Associate Professor, Division of Gastroenterology and Hepatology, Department of Internal Medicine, Nihon University School of Medicine. For ~25 years, he has focused his scientific interests on the topics related to liver diseases including acute liver failure, viral hepatitis and autoimmune liver diseases, and worked with Prof. Osamu Yokosuka. Tatsuo Kanda is also an expert for hepatitis A virus (HAV), HBV and HCV, and translation and replication of these viruses, and hepatocarcinogenesis. With his expertise in antiviral therapies and hepatitis virus research, Tatsuo Kanda also sees a lot of patients in clinical daily practice. Tatsuo Kanda has published more than 200 articles in peer-reviewed Journal.

Special Issue

Applications of Fluorescence in Situ Hybridization

Submission Deadline: July 31, 2018 (Open)                Submit Now

Guest Editor

Thomas Liehr, PhD, Dr.h.c., inv. Prof. (YSU and BMS)
University Clinic Jena, Institute of Human Genetics, Am Klinikum 1, 07747 Jena, Germany
Research Interests: clinical genetics; (molecular) cytogenetics; interphase architecture; ZOO-FISH; marker and derivative chromosomes

About This Topic

Fluorescence in situ hybridization (FISH) is an approach applied and applicable in many fields of biological research and diagnostics. Its unique feature is to provide information on localization and copy numbers of specific stretches of nucleic acids on the single cell level. Thus, FISH cannot be replaced by, like in medical field at present very popular ‘high throughput approaches’, which are mostly providing information on the genetic content of thousands to millions of cells at a time. FISH provides multiple possibilities and variants, according to the question to be answered. Fluorescence in situ hybridization can be applied as (i) single to multi-color FISH approaches, (ii) using DNA, RNA, cDNA, PNA, and other types of labeled nucleotides as probes; also (iii) samples for FISH can be various human, animal or plant derived tissue types, and even single cellular organisms.

In this special issue of OBM Genetics, we have selected topics that highlight the progress, the state-of–the-art and future potential of fluorescence in situ hybridization, ranging from practical and technological aspects to applications in research and diagnostics of fluorescence in situ hybridization in human, animal, plant, fungi, biofilms and bacteria. Overall this issue provides information where and how fluorescence in situ hybridization can be applied in practice, and shows its high potential in diagnostics and research.

Planned Papers

Title:Murine multicolor banding in solid tumor cell lines
Authors: Thomas Liehr 1,*, Eva Kubicova 1, Lisa-Marie Barf 1, Hans Rhode 1, Ralf Steinacker 1, Lioba Hergenhahn 1, Mattia Manferrari 1,2, Karolina Guja 1,3, Nadezda Kosyakova 1, Martina Rincic 4 , Shaymaa S. Hussein Azawi 1.
Affiliations: 1. Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany;
2. University of Trieste, Department of Life Sciences, Trieste, Italy;
3. Department of Plant Anatomy and Cytology Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland;
4. Department for Functional Genomics, Centre for Translational and Clinical Research, University Hospital Centre Zagreb, University of Zagreb School of Medicine, Zagreb, Croatia

Title: FISHing for an unstable cellular genome in the human brain
Authors: Yuri B Yurov 1,2, Svetlana G Vorsanova 1,2, Ivan Y Iourov 1,2,3
Affiliations: 1. Mental Health Research Center, Moscow, Russia;
2. Russian National Research Medical University named after N.I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russia;
3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health, Moscow, Russia

Title: FISH-based approaches for detecting mosaic aneuploidy
Authors: Svetlana G Vorsanova 1,2, Yuri B Yurov 1,2, Ilia V Soloviev 2, Alexei D Kolotii 1,2, Irina A Demidova 1,2, Viktor S Kravets 1,2, Oxana S Kurinnaia 1,2, Ivan Y Iourov 1,2,3
Affiliations: 1. Russian National Research Medical University named after N.I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russia;
2. Mental Health Research Center, Moscow, Russia;
3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health, Moscow, Russia

Title: Interphase quantitative FISH (IQ-FISH): implications for chromosome instability analysis
Authors: Ivan Y Iourov 1,2,3, Svetlana G Vorsanova 1,2, Ilia V Soloviev 1, Yuri B Yurov 1,2
Affiliations: 1. Mental Health Research Center, Moscow, Russia;
2. Russian National Research Medical University named after N.I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russia;
3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health, Moscow, Russia

Title: A case study of in vitro and in vivo karyotype evolution in melanoma using M-FISH
Authors: Banerjee R, Alifrangis C, Fu B, Corrie P, McDermott U and Yang F
Affiliations: Wellcome Trust Sanger Institute, Cambridge, United Kingdom; E-Mail:

Title: Fluorescence In Situ Hybridisation (FISH) is a useful tool in the detection of hypodiploidy in paediatric Acute Lymphoblastic Leukaemia (ALL)
Authors: Racha El-Hajj Ghaoui 1, Siobhan Battersby 1, Dorothy Hung 1, Bhavna Padhye 2, Dale C. Wright 1, 3
Affiliations: 1. Department of Cytogenetics, Sydney Genome Diagnostics, Sydney, Australia
2. The Cancer Centre for Children, Australia
3. Discipline of Child and Adolescent Health, University of Sydney, Sydney, NSW, Australia

Title: Colocalization of telomeric sequences and rDNA revealed by dual-FISH
Author: Susanna Salvadori
Affiliations: Dipartimento di Scienze della Vita e dell'Ambiente, Univ Cagliari, Dept Life & Environm Sci, Via T Fiorelli 1, I-09126 Cagliari, Italy

Title: Optimized Tools for Detection of Numerical Chromosome Aberrations Involving Chromosome 16 in Recurrent Pregnancy Complications
Authors: Kalistyn H. Lemke1, Anna R. Lawin-O'Brien2, Hui Zeng1, Jingly F. Weier1, 3, Mei Wang3, Heinz-Ulrich G. Weier1, Benjamin O’Brien4
Affiliation: 1. Life Sciences Division, University of California, E.O. Lawrence Berkeley National Laboratory, Berkeley, USA
2. Centre for Fetal Care, Queen Charlotte's and Chelsea Hospital, Imperial College Healthcare, London, UK
3. Dermatopathology Service, School of Medicine, University of California, San Francisco, USA
4. Department of Diabetes, Beckman City of Hope, Duarte, USA
5. William Harvey Research Institute, Queen Mary University London, UK, and Department of Anesthesiology, German Heart Institute, Berlin, Germany

Title: Usefulness of UroVysion test in diagnosis and monitoring of bladder cancer
Author: Edyta Borkowska
Affiliation: Medical University of Lodz, Chair of Laboratory and Clinical Genetics, Department of Clinical Genetics, Lodz, Poland

Title: The use of fluorescence in situ hybridization in domestic animals: practical applications in somatic and gamete cells
Author: Leopoldo Iannuzzi
Affiliation: Institute for Animal Production Systems in Mediterranean Environments (ISPAAM), National Research Council (CNR) of Italy, Via Argine 1085, 80147 Napoli, Italy

Title: A novel acquired t(2;4)(q36.1;q24) with a concurrent submicroscopic del(4)(q23q24) in an adult with polycythemia vera
Author: Eigil Kjeldsen
Affiliation: Professor and senior consultant, HemoDiagnistic Laboratory, Head of Cancer Cytogenetics Section, Aarhus University Hospital, Tage-Hansen Gade 2, Ent. 4A, DK-8000 Aarhus C, Denmark

Title: Interphase FISH: helpful assay in cytogenetics diagnosis
Authors: Elena Sala, Donatella Conconi, Nicoletta Villa, Gaia Roversi
Affiliation: U.S. di Genetica Medica, Ospedale San Gerardo

Title: The application of FISH in melanoma diagnosis
Author: Margit Balazs
Affiliation: Department of Preventive Medicine, Faculty of Public Health, University of Debrecen, Hungary, 4028, Debrecen, Kassai str. 26/b., Hungary

Title: FISH and SCLC targeted therapy
Author: Paul Hartel
Affiliation: Davis Medical Center of Davis Health System, Elkins, West Virginia, USA

Title: Biological effectiveness of alpha-particles: a cytogenetic evaluation by mFISH.
Author: Ritter S. et al
Affiliation: GSI Helmholtz Centre for Heavy Ion Research, Darmstadt, Germany

Title: The Application of FISH in our genetics practice: past and current
Authors: Dilys Andy, Rubin Wang
Affiliation: North East Thames Regional Genetics Service, Great Ormond Street Hospital for Children, Levels 4-6 Barclay House, 37 Queen Square, London WC1N 3BH, UK

Title: COMBO-FISH: A versatile tool beyond standard FISH to study chromatin organization by fluorescence light microscopy
Authors: Jin-Ho Lee, Matthias Krufczik, Eberhard Schmitt, Michael Hausmann


Technical Report

Quality Assurance/Quality Control of Fluorescence in Situ Hybridization Tests in Hematologic Malignancies

Zhenya Tang, Jun Gu, Guilin Tang, L. Jeffrey Medeiros
Received: July 22, 2018; Published: October 3, 2018; doi:10.21926/obm.genet.1804038

Original Research

Molecular Cytogenetic Characterization of Two Murine Colorectal Cancer Cell Lines

Hans Rhode, Thomas Liehr, Nadezda Kosyakova, Martina Rinčić, Shaymaa S. Hussein Azawi
Received: July 30, 2018; Published: September 20, 2018; doi:10.21926/obm.genet.1803037

Case Report

Identification of a Small Supernumerary Marker Chromosome Involving 11p14.1q12.1 in a Prenatal Case: Clinical and Molecular Characterization

Maria Kontodiou, Vassilis Paspaliaris, Themistoklis Dagklis, Elisavet Siomou, Ahmed Hamid Al-Rikabi, Kalliopi Tsita, Theano Stavroulaki, Andreas Pampanos, Apostolos Zavlanos, Georgios Papaioannou, Ioannis Papoulidis, Loretta Thomaidis, Emmanouil Manolakos
Received: May 30, 2018; Published: September 18, 2018; doi:10.21926/obm.genet.1803035

ase Report

sSMC Characterization in a Male with Turner Syndrome Stigmata

Frenny Sheth, Thomas Liehr, Kristin Mrasek, Joris Andrieux, Stuti Tewari, Naznin Lubna, Jayesh Sheth
Received: March 23, 2018; Published: September 07, 2018; doi:10.21926/obm.icm.1802033

Technical Report

User-Friendly Approach to Gain Isolation of Interphase Cells for Fluorescence in Situ Hybridization

Thomas Liehr, Monika Ziegler, Nadezda Kosyakova, Ahmed B.H. Al-Rikabi
Received: March 16, 2018; Published: May 18, 2018; doi:10.21926/obm.icm.1802021